中文摘要：

背景：大量体外筛选中得到的中药脑神经保护活性成分,多因脂溶性低难以透过血脑屏障而限制了其临床应用。目的：比较阿魏酸及其酯化产物阿魏酸甲酯、乙酯对原代培养的大鼠大脑皮质神经元体外存活的保护作用。方法：出生1d内的乳鼠采用酶消化法分离培养大脑皮质神经元。培养6d后,分别进行阿魏酸、阿魏酸甲酯及乙酯干预,继续培养1d。结果与结论：形态学观察结果显示细胞生长良好,NSE染色检查表明培养6d的存活细胞大部分均为神经元。MTT比色法测量结果显示,与空白对照组比较,阿魏酸仅在质量浓度为200mg/L时有明显促进大脑皮质神经元存活的作用,而阿魏酸甲酯、乙酯在质量浓度为0.16～20mg/L时均能明显促进神经元体外存活,且作用趋势、作用强度相近。表明阿魏酸的酯化产物阿魏酸甲酯和阿魏酸乙酯均能促进原代培养的大脑皮质神经元体外存活,显示出较好的脑神经元保护作用,且活性比阿魏酸更强。

英文摘要：

BACKGROUND：Many active components of traditional Chinese medicine of brain nerve protection screened in vitro have been limited in clinical application because of difficulties in passing through blood brain barrier due to low liposolubility.OBJECTIVE：To compare the protective effect of ferulic acid and its esterified products ferulic acid methyl ester and ethyl ester on in vitro survival of primary cultred cerebral cortex neurons.METHODS：Cerebral cortex neurons from rats at postnatal 1 day were isolated by enzyme digestion method.After 6 days of culture,these neurons were interfered with ferulic acid,ferulic acid methyl ester and ethyl ester and then cultured for 1 day.RESULTS AND CONCLUSION：Morphological results showed that cells well grew.Neuron specific enolase staining showed that after 6 days of culture,most of surviving cells were neurons.MTT examination results showed that compared with blank control group,200 mg/L ferulic acid could obviously promote the survival of cerebral cortex neurons,while 0.16-20 mg/L ferulic acid methyl ester and ethyl ester could obviously promote the in vitro survival of neurons,with similar tendency and strength.These results suggest that ferulic acid esterified products ferulic acid methyl ester and ethyl ester can promote the in vitro survival of primary cultured cerebral cortex neurons,exhibiting good protective effects on brain neurons,with stronger activity than ferulic acid.