甲醛已被广泛用于人们的日常生活中,成为室内环境的主要污染源。为探讨甲醛对动物不同发育阶段的毒性,本文以模式生物果蝇Drosophila melanogaster为对象,研究了饲料中添加不同浓度甲醛对果蝇生长发育的影响。结果显示,当对照组发育至3龄幼虫(孵化后3d)时,与未添加甲醛的对照组相比,饲料中添加0.25%或0.50%的甲醛极显著降低了幼虫的体重(P〈0.01),添加了0.50%甲醛的饲料组中发育出来的雌、雄成蝇体重也显著低于对照组,其中雌虫体重的差异达到极显著水平。此外,饲料中添加甲醛,还显著延长了幼虫发育的时间,即从胚胎到化蛹的发育时间、胚胎到羽化的发育时间都极显著长于对照组(P〈0.01),而其蛹期变态发育时间在对照组和各实验组之间没有显著差异。饲料中添加甲醛,还极显著降低了果蝇的产卵量(P〈0.01)。综上结果表明,饲料中含有甲醛可显著降低果蝇的发育速度,影响果蝇的生长和繁殖力,但对果蝇蛹期变态发育过程没有显著影响。
Formaldehyde is widely used in daily life nowadays.It has been the main source of environmental pollution caused by indoor decoration.To investigate the toxicity of formaldehyde on the development of different stages of Drosophila menlanogaster,we fed the flies with the diet containing formaldehyde and observed the situations of their development and reproduction.We showed that the contamination of 0.25% or 0.50% of formaldehyde resulted in significantly decreased body weight of the 3rd instar larvae(P0.01),3-d-old female adults(P0.01)and male adults(P0.05).In addition,feeding with formaldehyde contaminated medium caused significantly longer developing time from embryo to pupation than that in control(P0.01),while the time of metamorphosis in pupae was not impacted.Formaldehyde also brought the significantly decreased amount of spawned eggs(P0.01).These results suggest that formaldehyde may inhibit the growth of larvae and adults,prolong the developmental time of fly larvae and impact on their reproduction.