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中文摘要：
多聚半乳糖醛酸酶抑制蛋白（PGIP）是一种富含亮氨酸重复单位的糖蛋白,能非竞争性地抑制真菌多聚半乳糖醛酸酶（PG）的活性,从而提高植物的抗病性。以黄瓜、苦瓜、冬瓜、节瓜和甜瓜幼苗为材料,研究PGIP的诱导表达特性及在不同组织中的含量差异。通过比较经孢子诱导、SA诱导和黑暗诱导的黄瓜幼苗中的PGIP的相对含量,发现经病原菌孢子悬浮液处理48 h时PGIP的表达量最高。对经孢子诱导48 h的黄瓜幼苗的根、茎、叶中的PGIP含量进行比较,发现茎中PGIP的相对含量最高。测定这5种瓜类的PGIP对6种尖孢镰刀菌粗PG的抑制作用,发现苦瓜PGIP的相对活性较高,并证实了不同的PGIP能够特异性地识别不同的PG,PGIP对不同PG存在着选择性抑制。
英文摘要：
Polygalacturonase-inhibiting protein （PGIP） is a leucine-fich repeat cell wall-binding protein that can inhibit the activity of fungal PG specifically and non-competitively, thus improve the disease resistance of plants. Cucurbitaceae cucumis, Momordica charantia, Benincasa hispida, Benincasa hispida var. chieh-qua and Cucumis melo seedlings were selected in this research. Biotic stressed plants of C. cucumis showed high induced expression activity, rose up to maximum at 48 h and stems showed maximum expression than other organs. Biotic stressed plants of 5 kinds of cucurbits crops are used to prepare crude inhibitor and their activity was checked by inhibition assay to 6 kinds of PG respectively, PGIP of M. charantia showed high activity than others PGIP. The results showed a wide spectrum of activity of PGIP against different PG and there might be selective inhibition.
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