中文摘要：

目的：通过比较银屑病患者与对照组骨髓基质细胞生物学活性（包括细胞形态、免疫表型、增殖活性、自发凋亡率以及分泌细胞因子）的差异．揭示银屑病患者骨髓基质细胞的异常。方法：采用密度梯度离心法分别分离患者组与对照组骨髓单一核细胞，通过贴壁法培养骨髓基质细胞，收集传3代后的骨髓基质细胞及细胞培养上清液，流式细胞术鉴定其免疫表型及细胞凋亡率、四甲基偶氮唑蓝（MTT）比色法检测其增殖活性以及ELISA法检测细胞培养上清液中白介素（IL）-6、血小板源性生长因子（PDGF）和表皮生长因子（EGF）的水平。结果：患者组和对照组比较，骨髓基质细胞形态相似、表型相同，但细胞增殖活性、凋亡率以及细胞因子分泌水平均有差异（P〈0．05）。结论：银屑病患者骨髓基质细胞生物学活性异常，表明患者骨髓造血微环境可能存在异常。

英文摘要：

Objective： To reveal the abnormalities of bone marrow stromal cells in patients with psoriasis by comparing the biological activities （including morphology, immunophenotype, proliferative activity, apoptosis rate and cytokine secretion） in patients with psoriasis and normal controls. Methods： Bone marrow stromal cells from patients with psoriasis and normal controls were isolated by density gradient centrifugation method. The cells isolated were cultured,and cells at passage 3 and the culture supernatants were collected. The immuno-phenotype and spontaneous apoptosis rate of cells were analyzed by flow cytometry. The cell proliferation was measured by MTT colorimetric assay. ELISA was used to measure the concentrations of cytokines secreted from bone marrow stromal cells in the patients and the controls. Results： The cells from both groups had the same morphology and immunophenotype, but in comparison with the normal controls, the activity of proliferation of bone marrow stromal cells from the patients with psoriasis was significantly lower, the spontaneous apoptosis rate was significantly higher, the content of IL-6 in the culture supematant was significantly higher, and the content of PDGF and EGF was significantly lower（P〈 0.05）. Conclusion： The biological activities of bone marrow stromal cells from patients with psoriasis have abnormalities, which show that their hematopoietic microenvironment may be abnormal.