中文摘要：

目的：应用组织芯片技术分析抑癌候选基因NDRG2在人类甲状腺癌组织、甲状腺正常组织及甲状腺良性病变组织中的表达情况。方法：采用小鼠抗NDRG2单克隆抗体的免疫组织化学ABC法，研究NDRG2在不同甲状腺组织的表达，并分析NDRG2在不同甲状腺组织中的表达差异。结果：免疫组化结果显示，甲状腺癌组织与甲状腺良性病变组织中DNRG2的表达相比较呈显著性差异（P〈0．05），甲状腺癌组织与正常甲状腺组织中DNRG2的表达相比较呈显著性差异（P〈0．05）。甲状腺良性病变组织与正常甲状腺组织中DN—RG2的表达相比较呈显著性差异（P〈0．05）。结论：NDRG2在甲状腺癌组织中呈低表达，提示其可能对甲状腺癌的发生或发展有重要作用，这不仅为研究甲状腺癌的发病机制进一步提供了线索，而且对甲状腺癌的诊断和治疗具有重要意义。

英文摘要：

Objectire： By use of tissue microarray technique to analyse the expression of candidate tumor suppressor gene in human NDRG2 thyroid cancer,thyroid normal tissue and benign thyroid lesions. Methods：Immunohistochemical ABC method and image analysis were used to investigate the expression and content of NDRG2 in samples of human normal thyroid and carcinoma biopsies. Results ： Immunohistochemical results showed the expression of thyroid cancer and benign thyroid lesions in DNRG2 was significantly different （ P 〈 0.05 ） , DNRG2 expression in thyroid cancer tissue and normal thyroid tissue was significantly different （ P 〈 0.05 ）. DNRG2 expression in benign thyroid lesions and normal thyroid tissue was significantly different （ P 〈 0.05 ）. Conclusion ： NDRG2 in thyroid cancer was low expression, suggesting that in thyroid cancer development plays an important role, can be used not only to study the pathogenesis of thyroid cancer, but also for thyroid cancer diagnosis and treatment.