中文摘要：

Borf1 蛋白质被编码由一立即早牛的起泡沫的病毒(BFV ) 的基因；在病毒的生活环起一个关键作用。Borf1 是两个都装 transactivate 的 DNA 长末端重复(LTR ) ；BFV 由的内部倡导者(IP ) 对 transactivation 响应要素(TRE ) 明确地有约束力。分析潜水艇 Borf1 的细胞的本地化在 BFV 生活环期间，这基因被克隆进原核生物的表达载体；在一种可溶的形式表示了。在纯化以后；免疫，我们基于酶联免疫吸附测定结果与高滴定率提起了老鼠 anti-Borf1 浆液。西方的污点分析证明抗血清能明确地认出在 293T 房间被表示的 Borf1 蛋白质。与这特定的浆液，我们揭示了 nuclear；在是有 Borf1 的 transfected 的 HeLa 房间的 Borf1 的细胞质的本地化。而且，免疫荧光试金也出现了 Borf1 的本地化在感染期间；BFV 的转染是相同的。

英文摘要：

The Borfl protein is encoded by an immediate-early gene of the bovine foamy virus （BFV） and plays a key role in the viral life cycle. Borfl is a DNA binding protein which can transactivate both the long terminal repeat （LTR） and the internal promoter （IP） of BFV by specifically binding to the transactivation responsive element （TRE）. To analyze the subcellular localization of Borfl during the BFV life cycle, this gene was cloned into a prokaryotic expression vector and expressed in a soluble form. After the purification and immunization, we raised the mouse anti-Borfl serum with a high titer based on ELISA results. Western blot analysis showed that the antiserum could specifically recognize the Borfl protein that was expressed in 293T cells. With this specific serum, we revealed the nuclear and cytoplasmic localization of Borfl in HeLa cells that was transfected with Borfl. Moreover, the immuno-fluorescence assay also showed that the localization of Borfl during the infection and transfection of BFV was identical.