中文摘要：

目的探讨缺氧诱导因子-1α（HIF-1α）在前列腺癌发病机制中的作用。方法各取50例石蜡包埋的前列腺癌组织（前列腺癌组）和良性前列腺增生组织（良性前列腺增生组）,采用免疫组化法测定两组组织中HIF-1α蛋白的表达。结果前列腺癌组HIF-1α蛋白的阳性表达率和阳性表达强度均显著高于良性前列腺增生组（依次为：74.00%vs 12.00%;OD值为0.17±0.04 vs 0.14±0.03）（P〈0.01或P〈0.05）。前列腺癌组HIF-1α蛋白的阳性表达率低分化高于高、中分化（87.10%vs 52.63%,P〈0.05）,在伴有骨/淋巴结转移高于不伴有转移（81.58%vs 50.00%,P〈0.05）,而与TNM分期（Ⅲ~Ⅳ期和Ⅰ~Ⅱ期）、年龄（〉70岁和≤70岁）间差异无统计学意义（依次为：84.38%vs66.67%;75.86%vs 71.43%）（P〉0.05）。HIF-1α的阳性率在Gleason分级、TNM分期、骨及淋巴结转移间两两呈直线正相关（n=37,r=0.735、0.710、0.734,P〈0.01）。结论 HIF-1α在前列腺癌组织中表达增强,其可能参与了前列腺癌的形成过程,增强表达的HIF-1α蛋白可能与前列腺癌的恶化有关,预示预后不良。

英文摘要：

Objective To investigate the potential roles of hypoxia inducible factor（HIF）-1α in the pathogenesis of prostatic cancer. Methods HIF-1α protein were detected by immunohistochemistry techniques and analyzed in each 50 paraffin-embedded prostatic cancer and benign prostatic hyperplasia samples, named prostatic cancer group and benign prostatic hyperplasia group respectively. Results The positive expression rate and expression levels of HIF-1α in prostatic cancer group were significantly higher than those in benign prostatic hyperplasia group（74.00% vs 12.00%;optical density 0.17±0.04 vs 0.14±0.03, respectively）（P〈0.01 or P〈0.05）. Moreover, the positive expression rate of HIF-1αin prostatic cancer group with low differentiation and bone/lymph nodes metastasis were significantly higher than those with high or mode rate differentiation and without bone/lymph nodes metastasis（87.10% vs 52.63%;81.58% vs 50.00%,respectively）（P〈0.05）. Whereas, there was no statistic significance of HIF-1α positive reaction between Ⅲ-Ⅳ andⅠ-Ⅱ TNM grade or between 〉70 and ≤70 age（84.38% vs 66.67%;75.86% vs 71.43%, respectively）（P〉0.05）. Furthermore, the positive expression rate of HIF-1α in prostatic cancer among Gleason score and TNM grade and bone/lymph nodes metastasis were positive correlated（n=37, r=0.735, 0.710, 0.734, P〈0.01）. Conclusion The result shows that levels of HIF-1α expression are elevated at prostatic cancer tissue. These findings indicate that the HIF-1α system changes as prostate tissue progresses from a hyperplasia to a malignant state. Differential expression of certain HIF-1α system components in prostatic cancer may be associated with the malignant phenotype and more aggressive tumor behavior. Hence HIF-1α could serve to predict the harmful outcome of prostatic cancer.