中文摘要：

以＂圣斯威辛＂（Rosa‘St.Stwithum’）月季带腋芽的节为外植体,研究了＂圣斯威辛＂月季不定芽试管生根的最佳条件。结果表明：0.05mg/L NAA和3mg/L 6-BA的MS培养基中月季不定芽的诱导频率和平均每个外植体上的芽数最多,分别为95.7%和8.4个。将生长至3cm的不定芽转移到生根培养基上诱导根的形成,无激素处理的月季组培苗的平均每个不定芽生根数为6.3条,根诱导频率92%,平均根长为3.7cm。该结果与添加低浓度的IBA（0.2mg/L）对根的诱导效果没有显著性差异。高浓度的NAA和IBA诱导出大量的愈伤组织,不定根的数量显著减少。将生根的月季试管苗移栽到温室中,移栽成活率达到92%。

英文摘要：

Taking the axillary buds of Rosa ＇St.Stwithum＇ as explants,an efficient micropropagation system for Rosa ＇St.Stwithum＇ were studied.The results showed that 95.7% adventitious buds induction frequency and 8.4 buds per explant were achieved when cultured on MS media containing 0.05 mg/L NAA and 3 mg/L 6-BA.After transferred the adventitious buds,which length had grown to 3 cm,to rooting media,root organogenesis occurred.The control without any exogenous auxins treatment,which average roots number per stem,induction frequency and the average roots＇ length were 6.3 roots,92% and 3.7 cm,respectively,had no significant differences with 0.2 mg/L IBA treatments.High concentrations of NAA and IBA treatments induced a lot of callus,and significantly decreased adventitious roots.92% of the regenerated plantlets survived and grew vigorously in greenhouse condition.