欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
采用疏水性电荷诱导色谱（HCIC）新型生物分离方法，从CHO细胞培养液中高效分离抗HER2单克隆抗体（单抗）。选用典型HCIC介质MEP HyperCel，考察了细胞培养液中单抗稳定性，比较了不同pH下MEP HyperCel介质对抗HER2单抗的吸附性能，发现pH6～9范围内吸附容量均较高，酸性条件下吸附容量显著下降。进一步考察了抗HER2单抗的动态吸附载量，优化了上样和洗脱条件，确定了合适的分离条件，实现了细胞培养液中高效分离单抗，纯度达到94.6%，收率约0.1 mg·（ml料液）-1。结果表明，HCIC从哺乳动物细胞培养液中分离单抗是切实可行的，为单抗分离提供了新思路。
英文摘要：
Hydrophobic charge-induction chromatography （HCIC） is a new technology for biomolecule separation. In the present work HCIC was used to separate monoclonal antibody （mAb） anti-HER2 from Chinese Hamster ovary （CHO） cell culture broth with typical HCIC resin, MEP HyperCel. The stability of anti-HER2 mAb in cell culture broth was investigated firstly, and the adsorption of anti-HER2 mAb onto MEP Hypercellat different pH was compared. The adsorption capacities were high at the range of pH 6-9, but decreased significantly under acidic condition. The dynamic binding capacity of anti-HER2 mAb was determined with a packed bed, and the loading and elution conditions were optimized. The suitable separation conditions were obtained, and the purity of mAb could reach 94.6% with the yield of 0.1 mg·（ml broth）-1. It is feasible to separate mAb from mammalian cell culture broth with HCIC.
同期刊论文项目
同项目期刊论文
Preparation and characterization of supermacroporous polyacrylamide cryogel beads for biotechnologic
Evaluation of poly(ethylene glycol)/hydroxypropyl starch aqueous two-phase system for immunoglobulin
Preparation of ibuprofen-loaded chitosan films for oral mucosal drug delivery using supercritical so
Preparation of chitosan microparticles with diverse molecular weights using supercritical fluid assi
Isolation of immunoglobulin G from bovine milk whey by poly(hydroxyethyl methacrylate)-based anion-e
Enhancement of Laccase Activity by Marine-derived Deuteromycete Pestalotiopsis sp J63 with Agricultu
New hydrophobic charge-induction resin with 2-mercaptoimidazole as the ligand and its separation cha
Immiscible liquid-liquid slug flow characteristics in the generation of aqueous drops within a recta
Novel NaCS-CS-PPS microcapsules as a potential enzyme-triggered release carrier for highly-loading 5
Protein adsorption behavior and immunoglobulin separation with a mixed-mode resin based on p-aminohi
Effect and mechanism of sodium chloride on the formation of chitosan-cellulose sulfate-tripolyphosph
Co-expression of disulfide oxidoreductases DsbA/DsbC markedly enhanced soluble and functional expres
Evaluation of mixed-mode chromatographic resins for separating IgG from serum albumin containing fee
Evaluation and characterization of axial distribution in expanded bed. I. Bead size, bead density an
Effects of ligand density and pore size on the adsorption of bovine IgG with DEAE ion-exchange resin
Purification of Immunoglobulin of Egg Yolk with Hydrophobic Charge Induction Chromatography: Compari
Evaluation of a PEG/hydroxypropyl starch aqueous two-phase system for the separation of monoclonal a
Preparation and evaluation of dextran-grafted agarose resin for hydrophobic charge-induction chromat
Rapid freezing cryo-polymerization and microchannel liquid-flow focusing for cryogel beads: Adsorben
Adsorption of rutin with a novel beta-cyclodextrin polymer adsorbent: Thermodynamic and kinetic stud
Enhancing the activity and thermostability of thermostable beta-glucosidase from a marine Aspergillu
Formulation of Poorly Water-Soluble Compound Loaded Solid Lipid Nanoparticles in a Microchannel Syst
Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds fo
Microbial Side-Chain Cleavage of Phytosterols by Mycobacteria in Vegetable Oil/Aqueous Two-Phase Sys
Influences of Ligand Structure and pH on the Adsorption with Hydrophobic Charge Induction Adsorbents
Preparation of Cellulose Adsorbents with Ionic Liquid and Pore Expansion for Chromatographic Applica
Molecular recognition of Fc-specific ligands binding onto the consensus binding site of IgG: insight
An integrated expanded bed adsorption process for lactoferrin and immunoglobulin G purification from
Microchannel liquid-flow focusing and cryo-polymerization preparation of supermacroporous cryogel be
One-Step Purification of Lactoferrin from Crude Sweet Whey Using Cation-Exchange Expanded Bed Adsorp
Poly(hydroxyethyl methacrylate)-based composite cryogel with embedded macroporous cellulose beads fo
Separation of Recombinant Geranylgeranyl Diphosphate Synthase of Deinococcus radiodurans from Expres
A Novel Method for the Preparation of Spherical Cellulose-Tungsten Carbide Composite Matrix with NMM
期刊信息
位置：