中文摘要：

该研究采用RashidNp-nPP比色法，通过单因素实验和正交实验对可生物降解聚丁二酸丁二醇酯（PBs）的铜绿假单胞菌产脂肪酶条件进行了优化。研究结果表明：最适培养条件为：温度29℃，摇床转速120r／min；最佳培养基为：蔗糖0．5％，硫酸铵0．05％，硫酸‘亚铁0．005％，Tween一60与Span-80按l：l复配乳化剂0．5％，接种量5％，培养基初始pH9．0。优化后酶活可达33．74184U／mL。

英文摘要：

Rashid N p-nPP colorimetric method was used to demine the lipase activity, which was produced by Pseudomonas aeruginosa cultured under optimized conditions through mono-factor and orthogonal experiments. Results show the optimized conditions of culture as 29 ℃ and rotating speed 120 m; while the optimal culture media is sucrose 0.5%, （NI-L）2SO4 0.05%, FeSO4 0.005%, compound emulsifier （Tween--60 ： Span-80 as i ： 1 ）0.5%, seed volume 5%, and the initial pH 9.0. After optimization,the lipase activity is up to 33.741 84 U/mL.