中文摘要：

目的检测肺腺癌恶性胸腔积液中相关的miRNA表达，探讨miRNA表达与预后的关系。方法采用miRNA芯片技术筛选10例肺腺癌患者恶性胸腔积液miRNA表达谱，结合临床分析可能与预后有关的miRNA。采用实时荧光定量PCR（QPCR）技术检测84例肺腺癌恶性胸腔积液标本中miRNA表达，结合临床预后分析确认表达差异的miRNA。结果在5例无进展生存期（PFS）≥5个月和5例PFS≤2个月患者中，芯片筛查显示共13种miRNAs表达变化倍数〉2倍。在84例不同预后肺腺癌胸腔积液患者中选取miR．146a、miR-16、miR-155、miR-484、miR-134、miR-141、miR．106b和miR-93共8个miRNA进行QPCR验证，发现仅miR-93和miR-146a表达存在差异（P〈0．05），miR-93在预后好的患者中表达升高，变化倍数为2．41倍，miR-146a在预后好的患者中表达下降，变化倍数为0．46倍。结论miR-93在预后好的患者恶性胸腔积液中高表达，miR-146a则相反，其可能成为判断肺癌恶性胸腔积液患者预后新的分子标志物。

英文摘要：

Objective To investigate the expression of miRNA related to malignant pleura1 effusion in lung adenocarcinoma, and to investigate the relationship between the expression of miRNA and prognosis. Methods miRNA chip technique was used to screen the expression of malignant pleural effusion in 10 lung adenocarcinoma patients, and to analyze the possible correlation of miR- NA with clinical prognosis. The expression of miRNA was detected by quantitative real-time PCR （QPCR）in 84 cases of malignant pleural effusion of lung adenocarcinoma, and the differential expression of miRNA was confirmed by clinical prognosis analysis. Results In 5 patients of progression free survival （PFS） more than 5 months and 5 patients of PFS less than 2 months, microarray screening revealed a total of 13 miRNAs expression changing ratio〉2 times. Expression of miR-146a, miR-16, miR-155, miR-484, miR-134, miR-141, miR-106b and miR-93 was detected in 84 patients with pleural effusion of different prognosis. It was revealed that the expres- sion of miR-93 and miR-146a were different. Compared with patients with poor prognosis, the expression of miR-93 was increased by 2.41 times in patients with good prognosis; and the expression of miR-146a was decreased by 46%. Conclusion MiR-93 is highly ex- pressed and miR-146a is lowly expressed in malignant pleural effusion of patients with better prognosis. They may be new molecular markers for the prognosis of patients with malignant pleural effusion.