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中文摘要：
目的利用9.4T高分辨力MRS检测人脐带间充质干细胞（hUCMSCs）的水溶性及脂溶性提取物,明确其频谱代谢特征。方法培养获取hUCMSCs,利用甲醇/氯仿/水一次性提取细胞的水溶性及脂溶性代谢物,使用9.4T MR检测提取物的氢质子频谱,并定量计算谱线中主要代谢物的浓度。结果 hUCMSCs的水溶性与脂溶性提取物具有不同的频谱特征,水溶性提取物频谱中的主要代谢峰有乳酸、醋酸、苏氨酸、谷氨酸、肌酸、肌醇及胆碱复合物等,脂溶性谱线中主要代谢峰为长/短链脂肪酸等。结论 MRS能反映细胞的生理状态,综合分析两类提取物的频谱特征能更全面地获取间充质干细胞的代谢信息。
英文摘要：
Objective To get a more detailed understanding of the metabolic features of the human umbilical cord mesenchymal stem cells（hUCMSCs） by analyzing both the water-and lipid-soluble metabolite extractions with 9.4T MR spectroscopy（MRS）.Methods The hUCMSCs were cultured and collected,both water-and lipid-soluble metabolites were extracted simultaneously by a dual phase extraction procedure using methanol-chloroform-water and prepared for MRS analysis.1H proton spectra were acquired on 9.4T MR and the concentrations of main metabolites were quantitatively calculated.Results hUCMSCs had different spectral property between aqueous and organic metabolites.In the water-soluble metabolite profiles,main resonances were assigned to lactate,acetate,succinate,glutamate,creatine and choline-containing metabolites,while in the lipid-soluble metabolite profiles,most resonances were assigned to saturated and unsaturated fatty acids.Conclusion MRS is an effective tool to detect cell biological status.Combined analysis of both the water-and lipid-soluble metabolites enables an elaborate cognition of the metabolic profiles of hUCMSCs.
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