中文摘要：

采用荧光光谱法研究了中华蜜蜂化学感受蛋白3（CSP3）与其特异性配基N-苯基-1-萘胺（N-phenyl-1-naphthylamine,1-NPN）的相互作用.结果表明,1-NPN能使CSP3在λem=328 nm处发生荧光猝灭,且猝灭机理为静态猝灭.另外,猝灭过程的ΔH0〉0,ΔS0〉0,表明二者间的主要作用力为疏水相互作用.依据F rster非辐射能量转移机制得到二者的结合距离为9.3 nm,能量转移效率E=0.054.采用同步荧光光谱和圆二色光谱考察了1-NPN对CSP3构象的影响,CSP3的荧光主要来源于色氨酸残基,且最大发射波长略有红移,表明结合位点微环境的极性增加,且CSP3的α-螺旋比例相应减少,从而使CSP3构象发生变化.

英文摘要：

The molecular interaction of N-phenyl-1-naphthylamine（1-NPN） with purified recombinant Chemosensory proteins 3（CSP3） of Chinese honeybee,Apis cerana cerana,was studied by fluorescence spectra.By static quenching mechanism,1-NPN could quench the intrinsic fluorescence of CSP3 at 328 nm（λem）.Thermodynamic analysis show that hydrophobic interaction of 1-NPN with CSP3 was predominant intermolecular force.According to the Frster-type dipole-dipole nonradiative energy-transfer mechanism,the binding distance（r=9.3 nm） and energy-transfer efficiency（E=0.054） bewteen donor（CSP3） and acceptor（1-NPN） were obtained.Synchronous fluorescence spectra and circular dichroism spectra show that the tryptophan contributed primary fluorescent emission.The redshift of λmax and the reduction α-helix indicated that 1-NPN can affect the conformation of CSP3 by increasing the polarity of the microenvironment of binding sites between them.