中文摘要：

为筛选檀香心材总RNA提取方法，对5种提取方法进行比较研究，包括Trizol法、改良CTAB法、SDS酸酚法、异硫氰酸胍-CTAB法、异硫氰酸胍-SDS法。结果表明，Trizol法和异硫氰酸胍-CTAB法不能提取出檀香心材总RNA，而SDS酸酚法、改良CTAB法和异硫氰酸胍-SDS法均能提取檀香心材总RNA。SDS酸酚法的A260nm/A230nm小于2.0，且RNA产率低，仅为(27.94±1.06)μgg–1，不能满足后续实验要求。而改良CTAB法和异硫氰酸胍-SDS法提取的总RNA带型清晰,完整性好，A260nm/A280nm为1.8~2.0，A260nm/A230nm大于2.0，RNA产率分别为(79.06±4.22)和(107.00±1.36)μgg–1。分别以改良CTAB法和异硫氰酸胍-SDS法提取的总RNA为模板，通过RT-PCR反应，扩增檀香Actin基因片段，结果二者扩增产物大小相同且条带单一，说明改良CTAB法与异硫氰酸胍-SDS法为檀香心材总RNA提取的较好方法。

英文摘要：

In order to screen RNA extracting method from heartwood of Santalum album, five methods were compared, including Trizol method, improved CTAB method, SDS-phenol method, guanidine isothiocyanate-CTAB method, and guanidine isothiocyanate-SDS method. The results showed that it was difficult to extract totalRNA by Trizol method and guanidine isothiocyanate-CTAB method, and the improved CTAB method,SDS-phenol method and guanidine isothiocyanate-SDS method could successfully extract total RNA. TheSDS-phenol method was not suitable for RNA extraction, because A260 nm/A230 nm was less than 2.0 and total RNAyield was low with (27.94±1.06) μg g–1. However, the 28S rRNA and 18S rRNA bands of total RNA extracted byimproved CTAB method and guanidine isothiocyanate-SDS method were clear, A260 nm/A280 nm ranged from 1.8 to2.0, A260 nm/A230 nm were more than 2.0, the yields were (79.06±4.22) and (107.00±1.36) μg g–1, respectively. The Actin segments in total RNA extracted by improved CTAB method and guanidine isothiocyanate-SDS method could amplificated with the same size and sigle band. Therefore, the improved CTAB methodand and guanidine isothiocyanate-SDS method were effective methods to extract total RNA from heartwood of S. album.