中文摘要：

以H2O2-HAc体系下降解壳聚糖得到的甲壳低聚糖为原料，采用NF200-300卷式纳滤膜纯化除盐与聚丙烯酰胺凝胶（Bio-Gel P-2）层析分离相结合的方法，制备了高纯度的氨基葡萄糖和壳二糖。考察了纳滤的温度、压力与浓缩倍数对纯化效果的影响，结果表明：在0．4MPa、40℃、浓缩2倍的条件下，纯化效果最好，甲壳低聚糖中离子透过率为91．8％。同时考察了聚丙烯酰胺凝胶层析的接管时间（多长时间接一管）与上样质量浓度对纯化后甲壳低聚糖的分离效果的影响，结果表明：在接管时间为1min、上样质量浓度为0．2g／mL的条件下，分离得到了高纯度的氨基葡萄糖和壳二糖。使用MALDI-TOF-Ms对所得产物进行表征，并对所得产物的抗菌抑菌性进行了探究。

英文摘要：

Glucosamine and chitobiose were preparatd by NF200-300 nanofiltration and polyacrylamide gel chromatography（Bio-Gel P-2）,and chitooligosaccharide was used raw material which was prepared by oxidative degradation of chitosan with hydroperoxide in acetic acid system.The effects of the tem- perature,pressure and concentration multiple of nanofiltration on penetration ratio of ion were investi- gated. The optimum conditions were obtained as follows： nanofiltration pressure 0.4 MPa, nanofiltration temperature 40℃, concentration multiple 2.Under above conditions,penetration ratio of ion was 91.8%.And the effects of the time and sample concentration on separation of chitooligosaccha- ride were also investigated.Results showed that：time 1 min,sample concentration 0.2 g/mL,Under a- bove conditions, high-purity chitooligosaccharide monomer and chitobiose were preparated. The products were analyzed and characterized by MS, and the antibacterial properties of products were ex- plored.