试验采用营养液培养的方法,以玉米为试材,研究了不同供镉浓度(0、5、20和100μmol/L)和处理时间(12、2,4、48、96、168h)对植株体内钙调蛋白(CaM)含量及生物膜上的Ca^2++ATPase活性的影响。结果表明,植株可溶性Ca^2+含量在镉胁迫后较不加镉处理增加,镉处理在叶和根中分别在48和24h后达最高,然后随镉处理浓度和处理时间的增加逐步下降;同时镉诱导了植株CaM的合成,其含量随镉处理浓度和处理时间增加逐步增加,但20μmol/L和100μmol/L镉处理在168h后有所下降;与不加镉处理相比,镉胁迫导致植株生物膜上的Ca^2+-ATPase活性迅速升高,但随镉处理浓度提高和时间延长,镉胁迫植株的Ca^2+-ATPase活性在48h(质膜、液泡膜和内质网膜)和24h(线粒体膜)后逐步降低。各膜上的Ca^2+-ATPase活性依次为质膜〉液泡膜〉内质网膜〉线粒体膜,且同一微囊膜,根中的活性大于叶中。
A hydroponics culture experiment was conducted to examine the effects of cadmium (Cd) stress on calmodulin (CAM) content and Ca2^+-ATPase activities in maize (Zea mays) plant. Maize seedlings were treated with 0, 5, 20, and 100μmol/L Cd for periods of 12 to 168 h. Results showed that the content of soluble Ca^2+ in plant were increased by cadmium stress. The highest levels of soluble Ca^2+ in leaves and roots were found in the plants with 48 h and 24 h exposure to Cd respectively. CaM content in plants was enhanced with increasing Cd concentrations and prolonged Cd treatment. However, CaM content in plants began to decrease after 168 h treatments of 20μmol/L and 100μmol/L Cd. The Ca^2+ -ATPase activities of Cd stressed plants were higher than that without Cd addition, but they decreased gradually with higher concentrations of Cd and extended Cd treatment after 48 h (plasma, tonoplast and endoplasmic reticulum membrane) and 24 h (mitochondrial membrane). The decrease of Ca2^+ -ATPase activities in cell membrane systems showed the following pattern: plasma membrane 〉 tonoplast membrane 〉 endoplasmic reticulum membrane 〉 mitochondrial membrane. The Ca^2+ -ATPase activities of the same microsomal were higher in root than in leaf.