中文摘要：

目的：探讨盘状结构域受体2（DDR2）基因缺失小鼠的优化繁殖方法与子代小鼠DDR2基因型的鉴定方法,建立DDR2基因缺失小鼠模型,为进一步研究DDR2分子在肿瘤、肺纤维化、类风湿性关节炎等复杂疾病中的功能以及作用机制奠定基础。方法：将从美国JAX实验室引进的三对杂合子小鼠进行饲养并交配繁殖,繁殖成功后其子代中将会出现野生型、杂合子以及纯合子3种基因型。用基因组提取试剂盒试剂盒提取子鼠鼠尾的基因组DNA,经紫外分光光度计定量后,采用Taqman qPCR的方法准确鉴定出小鼠的基因型,同时观察子代小鼠各基因型的比例,并通过小鼠体态观察,进一步证实Taqman qPCR鉴定方法的可靠性。结果：DDR2基因杂合子小鼠互交繁殖可得到DDR2野生型小鼠、DDR2纯合子小鼠以及DDR2杂合子小鼠三种基因型小鼠,所得子代基本符合孟德尔遗传规律,且雌性和雄性DDR2纯合子小鼠体长均较DDR2野生型小鼠和DDR2杂合子小鼠小,鼻子也较其它基因型小鼠明显变短,无繁殖能力。依据Taqman qPCR的结果所得出的纯合子小鼠体貌特征与文献报道一致,证实了Taqman qPCR结果的可靠性。结论：雌、雄性DDR2杂合子小鼠交配可有效获得DDR2基因缺失小鼠;实验所用Taqman qPCR方法能够准确鉴定子代小鼠的基因型,DDR2纯合子小鼠的获得为后续实验的提供了较理想的动物模型。

英文摘要：

Objective： To explore the optimized methods of breeding and identification of Discoidin Domain Receptor 2（DDR2） gene deficient mice for laying a foundation for studying the role of DDR2 in tumor, fibrosis, rheumatoid arthritis, and other complex dis- eases. Methods： Breed and propagate the introduced heterozygote mice from JAX laboratory. Crosses between heterozygotes produced three genotype ofsprings： heterozygotes, homozygous and wild type. Genomic DNA extraction kit was used to extract the genome DNA from the tails of filial generation mice. After quantitated by UV spectrophotometer, Taqman qPCR method was employed to identificate murine gene type accurately and the proportion of offspring mice of each genotype was observed. To further confirme the reliability of Taqman qPCR method, the mice body were observed. Results： Crosses between heterozygotes produced a Mendelian distribution of three genotypes of mice： wild-type, heterozygous and homozygous, and the noses of DDR2 deficient mice are significantly shorter than other genotypes＇mice, and they were not able to reproduce. The aspectual features of homozygous mice obtained by Taqman qPCR results agree with those reported in the literatures, which further confirmed the reliability of the Taqman qPCR results. Conclusion： Through mating of heterozygous male and female mice, DDR2 gene-deficient mice were obtained efficiently; Taqman qPCR method can accurately identify DDR2 deficient mouse genotypes.