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中文摘要：
通过生物信息学方法预测了新城疫病毒（NDV）基质蛋白（M）的细胞核定位信号（NLS），用Overlap—PCR方法缺失NLS基因，分别构建M和M—ANLSEGFP重组质粒，转染单层鸡胚成纤维细胞（DF-1），激光共聚焦观察了M蛋白亚细胞定位。结果表明：M蛋白能进入宿主细胞核中，缺失NLS基因序列之后M蛋白只分布于细胞质中。结论：新城疫病毒M蛋白可以通过其核定位信号进入宿主细胞核。
英文摘要：
Based on bioinformatics method, the nuclear localization signal （NLS） of Newcastle disease virus （NDV） matrix protein （M） was predicted and deleted by an Overlap-PCR assay. Subsequently, the plasmids including the full-length M gene and M-△NLS EGFP gene were constructed, respectively, and were transfected into monolayer DF-1 cells growing on the glass slides. The subcellular localization of M protein was measured by a laser confoeal microscope. The results showed that the M protein entered into the nuclei with the help of NLS. However, M protein with the NLS deleted did not enter into the nuclei. Hence, the NLS is the key of matrix protein in nuclear entry.
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