中文摘要：

利用Au-S化学键的作用，在金基底上组装L-半胱氨酸（L-Cys），通过铜离子的配位作用，构建Cu^2／L-Cys／Au自组装膜．考察该自组装膜的组装条件、电化学特征、重现性与稳定性，研究不同浓度的L-色氨酸中膜的识别能力和识别选择性．结果表明，自组装膜具有对L-色氨酸识别的选择性，在不同浓度的L一色氨酸中，膜的电化学阻抗会发生明显变化．因此，利用该膜对L-色氨酸（L-Try）的选择性识别引起电化学阻抗变化的特性，可构建一种新型的免标记电化学阻抗法色氨酸传感器．

英文摘要：

With the help of chemical bonding of Au--S, L-cysteiney （L-Cys） was assembled onto the gold surface to form L-Cys/Au self-assembled monolayer. Taking advantage of coordinate action of Cu^2＋ with L-cysteine, the Cu^2＋/L- Cys/Au self-assembled membrane was constructed. The films assembling conditions; electrochemical characteristics, reproducibility and stability are discussed, and the reorganization selectivity for L-tryptophan （L-Try） are also studied. The experimental results showed that the film Cu^2＋/L-Cys/Au could selectively recognize for L-tryptophan. When Cu^2＋/L-Cys/Au acted on L-tryptophan, the electrochemical impedance changed, and the self-assembled membrane could selectively recognize L-tryptophan. A novel L-tryptophan sensor based on electrochemical impedance was built up.