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基于TGF-β1/Smads和ERK通路cross-talk研究新风胶囊改善佐剂性关节炎大鼠肺功能的机制
  • ISSN号:1673-1727
  • 期刊名称:《中华中医药杂志》
  • 时间:0
  • 分类:R285.5[医药卫生—中药学;医药卫生—中医学]
  • 作者机构:安徽中医药大学第一附属医院药学部,安徽合肥230031
  • 相关基金:国家自然科学基金青年基金(81403388);安徽省自然科学基金项目(1508085QH159);安徽中医学院临床科学研究基金项目(2012LC1-020B)
中文摘要:

目的:观察芝麻素对自发性高血压大鼠(SHR)心肌细胞凋亡的影响。方法:SHR大鼠随机分为SHR组、芝麻素高、低剂量组及卡托普利组,每组6只,另取WKY大鼠6只作为正常对照组。各治疗组分别灌胃给予相应药物14周。TUNEL染色法观察大鼠心肌细胞凋亡水平,Western blot法检测心肌组织Bax、Bcl-2蛋白表达水平,比色法检测血清乳酸脱氢酶(LDH)活性、心肌组织总抗氧化能力(TAOC)及丙二醛(MDA)含量。结果:与SHR组相比,各治疗组心肌细胞凋亡明显少(P〈0.05或P〈0.01),心肌组织Bax蛋白表达水平明显下调,Bcl-2蛋白表达水平明显升高。同时,各治疗组大鼠心肌组织MDA含量明显下降,T-AOC显著升高(P〈0.05或P〈0.01)。结论:芝麻素可显著减少自发性高血压大鼠心肌细胞凋亡,其机制与增强机体抗氧化能力、调节Bcl-2和Bax蛋白表达平衡有关。

英文摘要:

Objective: To observe the protective effect of sesamin on cardiomyocyte apoptosis in spontaneously hypertensive rats and the possible mechanism involved. Methods: Twenty -four spontaneously hypertensive rats (SHR) were randomly divided into SHR group, low dose sesamin group, high dose sesamin group and captopril group. Other 6 Wistar- Kyoto rats were used as the control group. Rats in sesanfin and captopril groups were given intragastrieally corresponding drugs for 14 weeks. Cardiomyocyte apoptosis was evaluated by TUNEL assay. Protein expressions of Bax and Bcl -2 were determined by western blot. Levels of serum activity of lactate dehydrogenase (LDH) and total antioxidant capacity (TAOC) and malondialdehyde (MDA) in cardiac tissue were determined by colorimetric assays. Results: Supplementation with sesamin significantly reduced cardiomyocyte apoptosis in SHR, with protein expression of Bax down - regulation and Bcl- 2 up -regulation markedly. In addition, sesamin effectively reduced serum level of LDH activity and enhanced T- AOC in cardiac tissue, while MDA content was reduced obviously( P 〈 0.05 or P 〈 0.01 ). Conclusion: Sesamin can suppress cardiomyocyte apoptosis in spontaneously hypertensive rats, of which the potential mechanism may be associated with the enhancement of antioxidant capacity and the regulation of the balance between Bcl -2 and Bax protein.

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期刊信息
  • 《中华中医药杂志》
  • 中国科技核心期刊
  • 主管单位:中国科学技术协会
  • 主办单位:中国中医药学会
  • 主编:佘倩
  • 地址:北京市和平街北口樱花路甲4号
  • 邮编:100029
  • 邮箱:
  • 电话:
  • 国际标准刊号:ISSN:1673-1727
  • 国内统一刊号:ISSN:11-5334/R
  • 邮发代号:18-90
  • 获奖情况:
  • 国家中医药管理局优秀期刊一等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,美国剑桥科学文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:36061