中文摘要：

建立了高效液相色谱一原子荧光分光光度法测定海产品中无机砷（AsV，AsⅢ）、有机砷（DMA，MMA，AsB）含量的方法。样品经含10％（体积分数）HCl的提取液振荡提取、离心分离、二路形态分析预处理、高效液相色谱分离，用原子荧光光度计检测As（Ⅲ），DMA，MMA，As（V）；四路条件（过氧化氢氧化和开启紫外灯）形态分析预处理装置处理，高效液相色谱分离，原子荧光光度计测定AsB。As（Ⅲ）线性范围为0—100．00μg／L，r20．9997；DMA线性范围为0～100．00μg／L，r2=0．9993；MMA线性范围为0-100．00／ag／L，r20．9990；As（V）线性范围为0—100．00μg／L，，=0．9991；AsB线性范围为0-200．00μg／L，r2=0．9994。3个样品加标回收率为As（Ⅲ）86．7％-89．4％，DMA111．2％～117．0％，MMA109．7％～111．6％，As（V）83．8％-90．7％，AsB88-3％～90．4％。用该方法测定虾仁（干）5个价态测定结果的相对标准偏差为3．07％-9．93％∽=6）。5个价态的检出限（S／N=2）为As（Ⅲ）0．29gg／L，DMA0．36gg／L，MMA0．27gg／L，As（V）0．56μg／L，AsB1．46μg／L。该方法适用于海产品中As（Ⅲ），DMA，MMA，As（V），AsB含量的测定。

英文摘要：

The method for the determination of inorganic arsenic（AsV, As Ⅲ ）, organic arsenic（DMA,MMA,AsB）in seafood by HPLC-AFS was established. The samples were surged and distilled with a mixed solution of 10% （volum ratio） HCl, separated in a centrifuge, then As（ Ⅲ ）, DMA, MMA, As（V）were determined by second road speciation analysis of pretreatment device and HPLC-AFS; AsB was determined by four road （ hydrogen peroxide oxidation and UV light was turned ） speciation analysis of pretreatment device and HPLC-AFS. The linear range was 0-100.00 μg/ L for As （Ⅲ ）, DMA, MMA,As（ V ）; 0-200.00 μg/L for AsB, and correlation coefficients r： were 0.999 7, 0.999 3, 0.999 0, 0.9991, 0.999 4 respectively. The three samples recoveries were 86.7%-89.4% for As（ Ⅲ ）, 1 11.2%--117.0% for DMA, 109.7%-111.6% for MMA, 83.8%-90.7% for AsV, 88.3%-90.4% for AsB. The relative standard deviation of determination results of shrimp （dry） samples of 5 valence states was 3.07%-9.93%（n=6）, and detection limit （S/N=2） of the method were 0.29,0.36,0.27, 0.56,1.46 μg/L for As（ Ⅲ ）, DMA,MMA, As（V）, AsB, respectively. The method is suitable for determination of As （Ⅲ ）, DMA, MMA, As （V）, AsB in seafood.