中文摘要：

收集松材线虫5虫株与拟松材线虫1虫株进行生物学杂交,结果显示：松材线虫各虫株均能与拟松材线虫杂交且杂交率均较高,但杂交后代中普遍存在线虫死亡率较高的现象。用杂交后代线虫与未杂交亲本线虫分别接种2年生马尾松苗,结果表明杂交后代线虫对接种松苗的致病性小于其未杂交亲本线虫,从接种后萎蔫的松苗中再分离线虫,杂交后代线虫再分离数远少于亲本线虫数。

英文摘要：

Bursaphelenchus xylophilus is different from B. xylophilus in pathogenicity and distribution. Whether the two species can be crossed and what is different in pathogenicity between the likely offspring and the parents are important information for taxonomy,epidemic,and control of the disease. The hybridization was conducted between 5 isolates of B. xylophilus from different countries and one isolate of B. mucronatus from China. The results showed that all nematode isolates of B. xylophilus were successfully crossed with the isolate of B. mucronatus and the rate of hybridization was relfative high,but parts of the hybrid offspring were not able to survive. The hybrid offspring and their parents were inoculated in pines of two-year-old Pinus massoniana. The results also showed that the pathogenicity of hybrid offspring was weaker than their parent nematodes,and the numbers of offspring nematodes reisolated from pines were significantly less than their parents.