中文摘要：

目的：观察糖肾方对C57BLKS/J db/db（db/db）小鼠肾脏损伤的保护作用,并探讨其机制。方法：8周龄雄性自发性2型糖尿病db/db小鼠与同源不发病db/m小鼠,随机分为正常对照组（db/m组）、模型组（db/db组）、糖肾方给药组（db/db＋TSF组）,连续喂养12周,记录体质量、肾重、血糖及24h尿白蛋白水平,观察肾脏病理改变,并检测肾组织NADPH氧化酶及Nrf2信号通路表达。结果：与db/m组相比,db/db组小鼠体质量、肾重增加,血糖升高,24h尿白蛋白维持在高水平（P〈0.01）,肾小球系膜基质增生;糖肾方干预后可降低db/db小鼠体质量、肾重及24h尿白蛋白水平（P〈0.05,P〈0.01）,改善肾组织病理改变,下调肾皮质Nox2、Nox4、p22phox、Nrf2、NQO1表达（P〈0.05,P〈0.01）,上调HO-1表达（P〈0.05）。结论：糖肾方对db/db小鼠肾脏具有保护作用,其机制可能与下调NADPH氧化酶表达从而改善氧化损伤有关。

英文摘要：

Objective： To observe the renoprotective effect and mechanisms of Tangshen Formula（TSF） on db/db mice. Methods： Eight-week-old male db/db mice and homology db/m mice with spontaneously type 2 diabetes mellitus were divided into normal control group（db/m group）, model group（db/db group） and TSF group（db/db＋TSF group） and fed for 12 weeks. Body weights, kidney weights, blood glucose levels, 24 h urine protein levels, morphology study and oxidative stress markers were detected. The expression of NADPH oxidase and Nrf2 signal path were detected. Results： Compared with db/m group, the db/db group showed higher body and kidney weights, higher blood glucose levels, higher 24 h urine protein levels（P〈0.01） and severe morphological changes; TSF treatment decreased body weights, kidney weights and 24 h urine protein levels（P〈0.05, P〈0.01）, alleviated morphological changes, suppressed the expression of Nox2, Nox4, p22 phox, Nrf2, NQO1（P〈0.05, P〈0.01）, upregulated HO-1 expression（P〈0.05）. Conclusion： TSF has renoprotective effect on db/db mice, and the mechanism might be related with reducing the expression of NADPH oxidase to suppress oxidative stress.