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中文摘要：
为构建带有荧光标签的汉滩病毒受体整合素β3与细胞因子TNFRⅠ的稳定转染细胞系,构建目的基因的真核表达载体pIRES2-EGFP-β3和pIRES2-DsRed-TNFRⅠ（EC）,并转染CHO细胞,直接荧光观察报告基因和荧光定量RT-PCR检测目的基因mRNA表达。结果转染重组质粒pIRES2-EGFP-β3的细胞可检测到荧光报告基因和目的基因β3 mRNA的表达,转染pIRES2-DsRed-TNFRⅠ（EC）的细胞可检测到荧光报告基因和目的基因TNFRⅠmRNA的表达。结果表明成功地构建了真核表达整合素β3与TNFRⅠ的稳定细胞系。
英文摘要：
To construct eukaryotic expression vector of hantaan virus（HTNV） receptor integrin β3 and potential factor TNFRⅠ,and to establish stable transfected CHO cell lines,β3 and TNFRⅠ were generated by PCR from plasmids constructed before,then inserted into eukaryotic expression vectors pIRES2-EGFP and pIRES2-DsRed.The recombinant vectors were transfected into CHO cells by FuGENE HD.After screen with G418,stable transfected CHO cell lines were established.The expression of target gene was identified by fluorescence and qRT-PCR.The results shoned that the eukaryotic expression vectors was constructed succefffully.And the stable transfected CHO cell lines was constructed.It is concluded that establishment of stable transfected CHO cell lines and the expression of the target genes provide foundation for further expermental studies.
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