中文摘要：

从连续生物制氢反应器中分离得到高效产氢细菌P22,利用微生物自动鉴定仪并结合菌株16S rDNA序列分析,将其鉴定为Clostridium butyricum P22。同时还进一步研究了碳源、氮源、初始pH值和培养温度对菌株P22产氢活性的影响。结果表明：该菌株能利用多种碳源氮源产氢,当以葡萄糖作为产氢底物时,其最大产氢量和产氢速率分别为为301.7 mL/g-glucose（2.42 mol/mol-glucose）、31.1 mL（/g-glucose.h）。发酵液的初始pH和培养温度严重影响产氢菌活性,初始pH6.47、35℃培养为该菌种较为适宜的产氢条件。利用Arrehnius方程以葡萄糖为底物拟合得到该菌株产氢反应的活化能为70.7 kcal/mol。经能量分析,Clostridium butyricum P22产氢过程中葡萄糖能量回收率为20.6%。该菌株在生物制氢中具有很好的应用发展前景。

英文摘要：

A hydrogen-producing strain newly isolated from anaerobic sludge in a continuously stirred anaerobic bioreactor was identified as Clostridium butyricum P22 by 16S rDNA gene sequence analysis and detection by BioMerieux Vitek.Effects of carbon sources,nitrogen source,initial pH and culture temperature on the hydrogen production with Clostridium butyricum P22 were also studied.Results showed that the strain could utilize various carbon and nitrogen source to produce hydrogen.The maximal hydrogen yield and hydrogen production rate were obtained as 301.7 mL/g-glucose（2.42 mol/mol-glucose） and 31.1 mL/（g-glucose·h） respectively.Initial pH 6.47 and culture temperature of 35 ℃ were established to be the optimal conditions for hydrogen production.Considering glucose as a substrate,the activation energy for hydrogen production was calculated as 70.7 kcal/mol and 20.6% of substrate energy was recovered in the form of hydrogen.Results indicated that Clostridium butyricum P22 is an ideal candidate for the fermentative hydrogen production.