中文摘要：

【目的】探讨上调microRNA-205（miR-205）表达对HeLa细胞生物学特性的影响。【方法】实验分4组：miR-205上调组,阴性对照组,空白转染组和空白对照组。每种检测重复3次。利用脂质体siPORT NeoFX Transfection Agent,向miR-205上调组细胞转染Pre-miR-hsa-miR-205 miRNA Precursor,阴性对照组细胞转染Cy3 dye labeled PremiR Negative Control。荧光显微镜下评估转染情况,实时定量PCR方法检测miR-205表达,四甲基偶氮唑蓝（MTT）法和平板克隆形成实验分别检测细胞生长和增殖能力,流式细胞术检测细胞凋亡率和细胞周期分布,Transwell小室法检测细胞迁移能力。【结果】实时定量PCR结果显示,miR-205上调组miR-205的表达增加3061.5倍。与空白对照组相比,72hmiR-205上调组与阴性对照组活细胞数分别为（126±41）%,（126±39）%;两组克隆形成率分别为（79±11）%,（63±10）%;凋亡率分别为（4.6±2.1）%,（4.1±2.4）%;S期细胞比例分别为（19.2±3.6）%,（23.4±3.0）%;200倍镜视野下迁移细胞数分别为63±17和68±16。与对照组比,miR-205上调组细胞增殖能力增强但生长曲线不受影响,S期细胞比例降低,而凋亡率不变,细胞迁移能力无明显改变。【结论】上调miR-205表达后,HeLa细胞增殖能力及细胞周期改变,但生长曲线、凋亡率和迁移能力无变化。

英文摘要：

[ Objective ] To investigate the effects of the upregulated expression of microRNA-205 （miR-205） on the biological properties of HeLa cells. [Methods] There were four groups： miR-205 up-regulation group, negative control group, mock transfection group, and blank control group. Each kind of detection was repeated for three times. Through siPORT NeoFX transfection agent, the ceils in miR-205 up-regulation group were transfected with Pre-miR-hsa-miR-205 miRNA precursor, while the cells in negative control group were transfected with Cy3 dye labeled PremiR negative control. The transfection efficiency was evaluated under fluorescence microscope. The miR-205 expression was measured by real time PCR. Cellular growth and proliferation capacity were assayed by the methyl thiazolyl tetrazolium （MTr） assay and colony formation assay, respectively. The apoptosis rate and cell cycle distribution were tested by flow cytometry. Cellular migration ability was detected by Transwell chamber assay. [Results] Real time PCR showed that the miR-205 expression in miR-205 up-regulation group increased 3061.5 folds after the transfection of hsa-miR-205 Precursor. Compared with that of blank control group, the numbers of live cells 72 h after transfection in miR-205 up-regulation group and negative control group were （126 ± 41）% and （126 ± 39）% respectively. In miR-205 up-regulation group and negative control group, the colony forming rates were （79 ± 11）% and （63 ± 10）% respectively, the apoptosis rates were （4.6 ± 2.1）% and （4.1 ± 2.4）% respectively, the cell proportions in S phase were （19.2 ± 3.6）% and （23.4 ± 3.0）% respectively, and the migration ceils under 200 magnification field were （63 ± 17） and （68 ± 16） respectively. The cell proliferation ability was enhanced in miR-205 up-regulation group, while the cellular growth activity did not change compared with that in control group. The cell proportion in S phase decreased, while the apoptosis rate and the cellular migrati