中文摘要：

利用冷冻切片和荧光显微技术检测了褐飞虱取食对不同耐虫性水稻叶鞘组织中胼胝质的分布、形态和数量的影响，发现胼胝质主要分布在韧皮部组织、薄壁组织、表皮组织和木质部组织中，尤其以韧皮部筛管中的胼胝质沉积最多；胼胝质的形态主要有丝状、颗粒状，尤其是小颗粒占大多数。在褐飞虱取食、人工针刺或者注射褐飞虱唾液后，不同耐虫性水稻叶鞘组织胼胝质的沉积数量和面积明显增加，尤其是感虫品种TN1增加显著。移除褐飞虱后稻株恢复生长8d，与处理前比较，感虫品种的胼胝质数量和面积无明显差异，耐虫品种UtriRajapan明显减少，抗生性品种RathuHeenati略有减少。停止人工针刺或注射褐飞虱唾液后稻株恢复生长8d，感虫品种的胼胝质数量和面积显著增加，耐虫品种无明显差异，抗生性品种有所增加。上述结果说明，品种耐虫性与叶鞘维管柬组织中胼胝质的沉积数量和沉积面积有关。

英文摘要：

Effect of feeding of brown planthopper （BPH）, Nilaparvata lugens （Stal）, on depositional site, shape and numbers of callose in various leafsheath tissues were investigated using frozen section and fluoresence microscopy. The results showed that callose was mainly deposited in phloem, parenchyma, epidermal and xylem tissues, with phloem being dominant. Most ealloses have a small granular shape. After treated with BPH feeding, manual acupuncture or injection of BPH saliva, numbers and distribution of callose in leafsheath increased evidently in each of the three rice varieties that have different tolerance, in particular in the susceptible variety TN1. As compared with healthy plants （control）, after eight days recovery growth from BPH removal, callose number and distribution did not change significantly in TN1 leafsheaths, reduced evidently in the tolerant variety Utri Rajapan, and reduced slightly in the antibiosis variety Rathu Heenati. By contract, in the treatments of manual acupuncture or injection of BPH saliva, numbers and distribution of callose after eight days of recovery growth were significantly increased in the susceptible variety, changed little in tolerant variety, and increased slightly in antibiosis variety, as compared with control plants. It was concluded that tolerance of rice varieties were related to abundance of callose deposited in phloem tissue of leaf sheath.