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内细胞团及滋养外胚层对体外受精单囊胚移植结局的影响
  • ISSN号:1002-0322
  • 期刊名称:《真空》
  • 时间:0
  • 分类:R689.2[医药卫生—骨科学;医药卫生—临床医学;医药卫生—外科学]
  • 作者机构:广西壮族自治区人民医院生殖医学与遗传中心,南宁530021
  • 相关基金:国家自然科学基金资助项目(编号:81360107); 广西卫计委科研课题(编号:Z2014193)
中文摘要:

目的探讨精子形态与精子动力学参数之间的关系。方法 149例精液标本,采用西班牙SCA计算机辅助精子分析系统(CASA)分析精子动力学参数,同时用Diff-Quik快速染色方法对精子进行形态染色,按《WHO人类精液检查与处理实验室手册》(第5版)标准进行精液常规和精子形态分析。精子形态正常组75例,异常组74例。结果精子形态正常组的曲线速率(VCL)、直线速率(VSL)、平均路径速率(VAP)、精子头侧摆幅度(ALH)和鞭打频率(BCF)均显著高于精子形态异常组(P〈0.01),而两组之间直线性(LIN)、摆动性(WOB)、前向性(STR)差异无统计学意义(P〉0.05)。形态正常精子百分率与VCL、VSL、VAP、ALH、BCF存在显著正相关性(P〈0.01);单纯头部畸形精子百分率与VCL、VSL、VAP、LIN、STR、ALH、BCF存在显著正相关性(P〈0.01);混合畸形精子百分率与VCL、VSL、VAP、LIN、STR、ALH、BCF存在显著负相关性(P〈0.01)。结论精子形态与精子运动速率学参数之间存在一定的相关性,精子形态异常影响精子的运动速率、空间位移程度和运动方式。单纯的精子头部畸形不是影响精子运动速率和空间位移程度的决定性因素。混合畸形可能是影响精子运动能力的主要原因之一。

英文摘要:

Objective To investigate the relationships between sperm morphology and sperm motility parameters. Methods One hundred and forty-nine semen samples were tested. Sperm motility parameters were analyzed by computer-aided sperm analysis( CASA),and the sperm morphology was observed by Diff-Quik rapid staining. Sperm morphology and motility were classified based on the "WHO laboratory manual for the examination and processing of human semen"( Fifth Edition). Some of the samples were morphologically normal( normal group,n = 75) and the others were morphologically abnormal( abnormal group,n = 74). Results VCL,VSL,VAP,ALH and BCF were significantly higher in the morphologically normal group than those in the abnormal group( P〈 0. 01),but there were no significant differences in LIN,WOB and STR between the two groups( P〈 0. 05). There were significantly positive correlations between the morphologically normal sperm rates and VCL,VSL,VAP,ALH,BCF. The percentage of the sperms that only had the abnormal heads was positively correlated with VSL,VAP,VCL,LIN,STR,ALH and BCF. The percentage of multiple abnormalities sperms was negatively correlated with VSL,VAP,VCL,LIN,STR,ALH and BCF. Conclusion There are correlations between sperm morphology and sperm motility parameters. Abnormal sperm morphology influences the movement velocity,the space displacement and the movement way of sperms. The sperms with only head deformity are not the decisive factors affecting the sperm motility rate and the degree of space displacement. Multiple abnormalities may be one of the main reasons affecting sperm motility.

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期刊信息
  • 《真空》
  • 北大核心期刊(2008版)
  • 主管单位:
  • 主办单位:中国机械工业集团公司有限公司 沈阳真空技术研究所
  • 主编:李玉英
  • 地址:沈阳市沈河区万柳塘路2路
  • 邮编:110042
  • 邮箱:zkzk@chinajournal.net.cn
  • 电话:024-24121929
  • 国际标准刊号:ISSN:1002-0322
  • 国内统一刊号:ISSN:21-1174/TB
  • 邮发代号:
  • 获奖情况:
  • 工程技术类核心期刊,中国期刊网、光盘国家工程中心用刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2000版)
  • 被引量:3452