中文摘要：

旨在研究脂多糖（LPS）对绵羊输卵管上皮细胞β-防御素-1（SBD-1）表达的影响及其调控途径,本研究设置不同浓度（10ng·mL^-1、50ng·mL^-1、100ng·mL^-1、200ng·mL^-1、1mg·mL^-1）LPS,分别作用不同时间（0、1、3、6、12和24h）,检测绵羊输卵管上皮细胞SBD-1和Toll样受体-4（TLR4）mRNA表达水平,通过免疫组化检测SBD-1表达定位,并进一步通过TLR4阻断试验来证实TLR4介导LPS引起SBD-1表达。结果表明,SBD-1蛋白表达于输卵管上皮细胞。LPS以浓度和时间依赖方式影响绵羊输卵管上皮细胞中SBD-1的表达,且100ng·mL^-1 LPS在作用12h后,SBD-1的表达水平达到最高。阻断试验表明,TLR4介导LPS对SBD-1的表达调控。综上表明,LPS可以影响绵羊输卵管上皮细胞表达SBD-1,且该过程通过TLR4介导。

英文摘要：

The aim of this study was to explore the effect of lipopolyscharride（LPS）on induction of sheep beta defensin-1（SBD-1）in ovine oviduct epithelial cells（OOECs）.In this study,OOECs were cultured and then exposed to LPS for up to 24 hours,with a range of concentrations（10ng·mL^-1-1mg·mL^-1）.qRT-PCR was performed to detect SBD-1mRNA.The results showed that LPS stimulated the expression of SBD-1in a time-（maximal at 12hours）and concentration-（maximal at 100ng·mL^-1）dependent manner.Moreover,IHC staining showed that SBD-1was mainly expressed in epithelial cells.Importantly,TLR4 was detected in OOECs and stimulated by LPS.LPS induced SBD-1mRNA expression was markedly inhibited by blockage of TLR4 activity using anti-TLR4 antibody.In conclusion,these results suggest that LPS might induce the expression of SBD-1in the ovine oviduct epithelial cells,and the expression are mediated by TLR4.