中文摘要：

进行了Anabaena sp.PCC7120的别藻蓝蛋白ApcE与ApcF的体内重组的光谱和apcE和apcF基因的细菌双杂交的研究。在提纯前PCB-ApcE/PCB-ApcF的吸收及荧光光谱峰与PCB-ApcF的峰位置一致,而提纯后PCB-ApcE/PCB-ApcF的吸收及荧光光谱峰与PCB-ApcE的峰位置一致,表明ApcE与ApcF不能形成复合物。pBT-apcF与pTRG-apcE的转化细胞能在无3氨基-1,2,4三氮唑（3-AT）的非选择性培养基上生长,而不能在有3AT的选择性培养基上生长,说明在转化过程中未产生能抗3-AT的HIS3报告基因,进一步证实ApcE与ApcF间没有相互作用。

英文摘要：

We conducted a study on allophycocyanin proteins ApcE and ApcF from Anabaena sp.,PCC7120 bound PCB in Escherichia coli and two-hybrid bacteria system of ApcE and ApcF.Results showed that the λmax of absorption and fluorescence spectra of PCB-ApcE/PCB-ApcF were the same as the λmax of PCB-ApcF before purification,and the same as the λmax of PCB-ApcE after purification,indicating that ApcE and ApcF could not form a compound.While the transcription of pBT-apcF and pTRG-apcE could grow in the non-selective growth medium,they could not grow in the selective growth medium containing 3-amino-1,2,4-triazole（3-AT）.The HIS3 reporter gene did not engender during the transcription,indicating there were no interactions between ApcE and ApcF.