中文摘要：

目的：探讨高迁移率族蛋白A2（HMGA2）在肿瘤发生和血管形成中的作用。方法：采用Clontech公司软件设计靶向人HMGA2基因的5条小干扰RNA（siRNA）序列,常规培养肿瘤细胞系和血管内皮细胞系,利用脂质体2000将HM-GA2 siRNA导入细胞,通过MTT方法和Tran-swell模型检测细胞的增殖和迁移能力,采用小管形成实验检测血管内皮细胞的小管形成能力,荧光定量PCR方法检测细胞内的HMGA2 mRNA的变化。结果：5条siRNA中,HMGA2 siR-NA1、3、5不但能抑制肿瘤细胞的增殖,还可抑制血管内皮细胞的增殖、迁移和小管形成,尤其以HMGA2 siRNA5最为明显,处理组细胞的相对增长率分别为：Hela 63.2%±6.5%（P〈0.01）;人肺腺癌SPC-A1,86.8%±3.5%（P〈0.01）;人脐静脉内皮细胞（ECV-304）,58.5%±6.3%（P〈0.01）;人膀胱上皮细胞株（HUVEC）,60.3%±7.3%（P〈0.01）。HMGA2 siRNA5抑制两种血管内皮细胞的迁移（P〈0.01）,并降低HM-GA2基因的表达（P〈0.01）。结论：HMGA2除了在肿瘤发生过程中起作用之外,在血管形成中同样具有重要作用。

英文摘要：

AIM： To analyze the roles of high mobility group A2 protein（HMGA2） in tumorigenesis and angiogenesis.METHODS： 5 siRNAs targeting HMGA2 gene（HMGA2 siRNA1-5） were designed,synthesized,and transfected into cells using Lipofactamine 2000.MTT assay,transwell assay and tube formation assay were applied to evaluate the effects of these siRNAs on cancer cells（SPC-A1 and Hela） and endothelial cells（ECV-304 and HUVEC）.RESULTS：HMGA2 siRNA1,3 and 5 inhibited the growth of all the cell lines that were tested.Among these siRNAs,HMGA2 siRNA5 show the best inhibition effect.The relative growth ratios were 63.2%±6.5%（P0.01）,86.8%±3.5%（P0.01）,58.5%±6.3%（P0.01）,and 60.3%±7.3%（P0.01） for Hela,SPC-A1,EVC-304 and HUEVC cells,respectively.Furthermore,interfering the expression of HMGA2 affected the growth,migration,and tube formation of endothelial cells（P0.01）.CONCLUSION： HMGA2 protein plays important roles both in tumorigenesis and angiogenesis.