以平均体重为(8.13+0.05)g的鲈鱼(Lateolabraxjaponicus)幼鱼为研究对象,探讨了在低鱼粉饲料中添加牛磺酸、蛋氨酸和半胱氨酸对鲈鱼幼鱼生长及鱼体氨基酸组成的影响。分别在基础饲料中添加0(对照组T-0)、1.0%牛磺酸(T-1)、2.0%牛磺酸(T-2)、0.5%蛋氨酸(M-0.5)和0.5%半胱氨酸(C-0.5)制成5种等氮等脂的实验饲料,在室内流水养殖系统中进行为期70d的养殖实验。结果显示,T-1、T-2、M.0.5和C-0.5组鲈鱼幼鱼的终末体重、特定生长率(SGR)、增重率(WGR)和摄食率(FI)均显著高于T-0组(氏0.05);饲料中添加牛磺酸、蛋氨酸和半胱氨酸均可提高鱼体粗蛋白和粗脂肪含量(尸〈0.05),鱼体水分含量则呈现出相反的变化趋势;T-1、T-2组肝脏、肌肉中的牛磺酸含量显著高于T-0组(P〈0.05),但M.0.5、C.0.5组肝脏、肌肉中牛磺酸含量与T-0组无显著差异胗0.05);T-1、T-2和C.0.5组肝脏的必需氨基酸及总氨基酸含量均高于T-0组(P〈0.05),但M.0.5组肝脏必需氨基酸及总氨基酸含量与T-0组无显著差异(p0.05):T-1、T-2、M-0.5和C-0.5组肌肉的必需氨基酸含量均高于T-0组,但只有M-0.5组显著高于T-0组沪〈0.05);T-1、T-2和M.0.5组肌肉的总氨基酸含量高于T-0组(P〈0.05),C.0.5组与T-0组无显著差异(P〉O.05)。研究表明,饲料中添加牛磺酸、蛋氨酸和半胱氨酸均可提高鲈鱼幼鱼的生长,同时可以改善鲈鱼肝脏和肌肉中的氨基酸沉积。
A 10-week feeding experiment in indoor flowing-through water system was conducted to investigate the effects of dietary taurine, methionine and cysteine on growth and amino acid composition of Japanese seabass [mean initial weight (8.13±0.05) g]. A basal diet (as control) was formulated with 15% fish meal, 30% soybean meal, 13% corn gluten meal and 12% wheat gluten. The four experimental diets were supplemented with 1.0%, 2.0% taurine, 0.5% methionine and 0.5% cysteine (named as T-1, T-2, M-0.5 and C-0.5, respectively) to the basal diet to formulate isonitrogenous and isolipidic diets. Triplicate groups of 25 fish were fed to apparent satiation twice daily. During the experimental period, water temperature ranged in 23.2-28.8℃, and the dissolved oxygen content was approximately 7 mg/L. The results showed that the final body weight (FBW), specific growth rate (SGR), weight gain (WGR) and feed intake (FI) of T-1, T-2, M-0.5 and C-0.5 groups were significantly higher than those in the control group (P〈0.05). Fish fed the diets with taurine, methionine and cysteine supplementation had significantly higher whole-body crude protein and crude lipid contents (P〈0.05), while the whole-body moisture followed the opposite pattern. The taurine contents of liver and muscle in T-1 and T-2 groups were significantly higher than that in T-0 group (P〈0.05), however, the taurine contents of liver and muscle in M-0.5 and C-0.5 groups showed no significant difference with T-0 group (P〉0.05). Compared to the control, fish in T-I, T-2 and C-0.5 groups had higher liver essential amino acids and total amino acids contents (P〈0.05), while these values were not significantly different in the M-0.5 group (P〉0.05). The muscle essential amino acids content of the M-0.5 group was significantly higher than that of T-0 group (P〈0.05), while fish in T-I, T-2 and M-0.5 groups had significantly higher muscle total amino acids contents than T-0 group (P〈0.05). It is concl