中文摘要：

目的以人参皂苷Rh4（GRh4）和Rk3（GRk3）的生成量作为考察指标,利用响应曲面法（RSM）优化GRh4和GRk3的制备工艺。方法利用HPLC测定GRh4和GRk3的量,以人参皂苷Rg1（GRg1）为底物,采用高温热裂解,对影响热裂解的酸浓度、反应温度及时间进行考察,运用RSM选择最优GRh4和GRk3的热裂解工艺条件。结果经HPLC测定,根据Design Expert 7.1.6软件预测经高温热裂解GRg1制备GRk3和GRh4的最佳工艺参数：甲酸浓度为0.02%,反应温度为116.4℃,反应时间为2.22 h;在此条件下GRk3和GRh4之和最大为123.56 mg,得率为61.78%。结论 RSM优化后的高温热裂解条件,适合大规模制备GRk3和GRh4。

英文摘要：

Objective To employ the response surface methodology（RSM） to optimize the preparation conditions for ginsenoside Rh4（GRh4） and Rk3（GRk3） using the yields as index. Methods High temperature hydrolysis combined with RSM was used to prepare GRk3 and GRh4 from ginsenoside Rg1（GRg1）, and the reaction conditions were optimized at the same time. Results The optimal preparation conditions of GRk3 and GRh4 were confirmed using Design Expert 7.1.6 software as followss： acid concentration of 0.02%, reaction temperature of 116.4 ℃, and reaction time of 2.22 h, respectively. Under the above conditions, the yields of GRk3 and GRh4 were 123.56 mg with conversion rate of 61.78%. Conclusion The preparation conditions obtained from RSM optimization are suitable for large scale preparation of GRk3 and GRh4.