[目的]研究养分缺乏下外源有机酸对暗棕壤脱氢酶活性的影响。[方法]用不同比例 A1和 B层暗棕壤( A1∶B=1∶2)设置土壤养分缺乏条件、栽植落叶松幼苗,通过有机酸溶液外源添加,系统研究不同浓度草酸、柠檬酸和琥珀酸对养分缺乏下暗棕壤脱氢酶活性的影响。[结果]养分缺乏下,暗棕壤的脱氢酶活性明显降低,且胁迫时间越长降低幅度越大。对于养分缺乏的暗棕壤,大多数有机酸处理均提高了土壤的脱氢酶活性,提高程度因处理时间、有机酸种类和浓度而异。在处理10、20和30 d时,最显著的有机酸处理浓度分别为10.0、5.0和10.0 mmol/L;不同处理时间内对脱氢酶活性的增幅为30 d〉20 d〉10 d;3种有机酸的增加效果为:琥珀酸〉柠檬酸〉草酸。[结论]外源有机酸对养分缺乏暗棕壤的脱氢酶活性提高有很好的促进作用,也一定程度上提高了暗棕壤的微生物活性和土壤肥力。
Objective] This study aimed to investigate the effects of exogenous or-ganic acids on dehydrogenase activity in dark brown forest soils under nutrient defi-ciency. [Method] Different proportions of A1 and B horizon dark brown forest soils (A1∶B=1∶2) were utilized to establish soil conditions with nutrient deficiency for cultivation of Larix olgensis seedlings. The effects of oxalic acid, citric acid and succinic acid on dehydrogenase activity in dark brown forest soils under nutrient deficiency were studied systematical y by adding different concentrations of organic acid solutions. [Result] Under nutrient deficiency, dehydrogenase activity in dark brown forest soils was reduced significantly, and the reduction increased with the extension of stress duration. Most organic acid treatments improved dehydrogenase activity in nutrient-deficient dark brown forest soils, and the effects varied with different treatment du-ration and types and concentrations of organic acids. Furthermore, 10.0, 5.0 and 10.0 mmol/L organic acid treatments exhibited the most significant effects on day 10, 20 and 30, respectively. The increment of dehydrogenase activity in different durations showed a downward trend of 30 d〉20 d〉10 d; the improvement effects of three organic acids on dehydrogenase activity showed a downward trend of succinic acid〉 citric acid〉oxalic acid. [Conclusion] Exogenous organic acids improved signifi-cantly dehydrogenase activity in dark brown forest soils under nutrient deficiency and also improved the microbial activity and soil fertility to a certain extent.