中文摘要：

木糖醇亚硒酸酯是一种新合成的化合物,其能够引发人肝癌细胞系SMMC-7221细胞凋亡并且其引发SMMC-7221细胞凋亡伴随着谷胱甘肽的消耗.为了提供更多关于木糖醇亚硒酸酯的抗癌机制研究,该实验用荧光定量PCR技术检测经该化合物处理后的SMMC-7221细胞中caspase-3、caspase-8、caspase-9这3个基因mRNA水平的变化.在经0.5mg/L或1mg/L木糖醇亚硒酸酯分别处理SMMC-7221细胞12、24、36、48h后,与对照组相比,caspase-3的mRNA表达水平从12~48h内显著上调;此外,caspase-8和caspase-9的mRNA表达水平仅在24h显著变化,而在其他时间点与对照组相比变化不明显.与对照组相比,caspase-3、caspase-8、caspase-9的mRNA表达在12h上升,在24h到达最大值,在36h和48h逐渐下降.木糖醇亚硒酸酯作为一个能够诱导细胞凋亡的有应用前景的抗癌药物,可能是通过内源性和外源性信号通路引发SMMC-7221细胞凋亡,其深层次的抗癌机制需要更进一步的研究.

英文摘要：

Xylitol selenite, a novel synthetic compound, which is able to cause apoptosis in human hepatoma cell line SMMC-7221 cells and induce apoptosis following GSH depletion. To provide more investigation on its anti-cancer mechanisms, the changes of caspase-3, caspase-8 and caspase-9 in mRNA levels in SMMC-7221 cells treated with xylitol selenite were determined using real-time quantitative PCR. After treated with 0.5 or 1 mg/L xylitol selenite for 12, 24, 36, and 48 h, significant upregulation in the mRNA expression of caspase-3 was observed, comparing with control group. Moreover, expressions of both caspase-8 and caspase-9 mRNA only markedly varied in 24 h, and there were no significant differences at other time points. In contrast to control group, expressions of caspase-3, caspase-8 and caspase-9 mRNA were upregulated at 12 h, reaching its peak at 24 h and beginning to decrease at 36 and 48 h after xylitol selenite treatment. Xylitol selenite, a promising anti-cancer drug to induce apoptosis in SMMC- 7221 cells, may regulate the apoptosis through extrinsic and intrinsic pathway and more studies on its anti-cancer mechanisms should be determined.