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邻苯二甲酸酯对土壤微生物群落多样性的影响

ISSN号：0250-3301
期刊名称：《环境科学》
时间：0
分类：X171.5[环境科学与工程—环境科学]
作者机构：[1]山东大学环境研究院,济南250100, [2]山东大学环境科学与工程学院,济南250100
相关基金：国家自然科学基金重点项目（20737001/B0705）;中国博士后科学基金项目（10000059311025）

关键词：邻苯二甲酸酯, BIOLOG, 限制酶多态性分析, 群落, phthalic acid easters（PAEs） , BIOLOG, ARDRA, community

中文摘要：

邻苯二甲酸酯（PAEs）是存在最广泛的有机有毒污染物之一.利用BIOLOG和ARDRA方法,分别考察了不同浓度PAEs对土壤微生物代谢多样性和遗传多样性的影响.结果表明,不同浓度PAEs处理的土壤的平均颜色变化率（AWCD）值随时间增加的幅度不同,PAEs浓度越高、AWCD值增幅越小,对微生物代谢活性的抑制作用越大;不同浓度PAEs处理样品中微生物群落生理代谢活性和碳代谢类群表现出明显差异,空白和低浓度土壤微生物以糖类和羧酸类代谢群为优势类群,而中浓度和高浓度土壤微生物以多胺类代谢群为优势类群;主成分分析也显示碳代谢类型出现差异,PC1就能将4个样品很好地分开,与PC1正相关程度较高的碳源有L-天冬酰胺酸、4-羟基苯甲酸和D-苹果酸,负相关的碳源有D-半乳糖醛酸、i-赤藻糖醇、γ-羟基丁酸和1-磷酸葡萄糖.ARDRA带型分析表明不同的土壤样品的多样性指数随着PAEs浓度的增加而增加,短时间内PAEs能增加土壤微生物群落的代谢多样性.

英文摘要：

Phthalic acid easters （PAEs） are one of the most extensive organic and poisonous pollutants. BIOLOG and ARDRA method were used to analyze the impact of PAEs on microbial diversity in soil. BIOLOG analysis shows that the increase of average well color development （AWCD） is different in soils dealt with different concentrations of PAEs. With a higher PAEs concentration, the AWCD increases more slowly and the metabolic activity decreases more quickly. Physiological metabolism activity and carbon metabolic group show obvious difference in different samples. Although metabolic groups of carbohydrates and carboxylic acids were the dominant groups in control sample and low concentration sample, the dominant group is main polyamines in middle and high concentration samples. Principal component analysis （PCA） shows the differences of carbon metabolism. Four samples could be separated by PC1. Substrates of high positive correlation coefficients with PC1 were L-asparagine, 4-hydroxy benzoic acid and D-malic acid, but D-galacturonic acid, i-erythfitol, 7-hydroxybutyric acid and glucose-I- phosphate showed high negative correlation. ARDRA analysis showed that the diversity index decreased, with the increased concentration of PAEs. In a short time, PAEs could increase the diversity of microbial genotype in soil.