中文摘要：

目的探讨肾脑复元汤联合人脐带间充质干细胞（HUC-MSCs）移植对脑缺血损伤的神经保护机制。方法制作雄性SPF级Sprague Dawley大鼠大脑中动脉闭塞（MCAO）模型108只,随机分为3组,即模型组36只、干细胞组36只、联合组36只,各组再根据处死时间分为3 d、7 d、14 d 3个亚组;观察各组大鼠神经功能缺损评分,以及用免疫组织化学染色以及Western blot法检测脑缺血边缘区血管内皮生长因子（VEGF）的表达。结果 1）干细胞组和联合组在第3天、7天、14天的神经功能缺损评分均低于同时段模型组（均P〈0.01）;联合组在第7、14天神经功能缺损均低于同时段干细胞组（P〈0.05或P〈0.01）,联合组和干细胞组在第3天的神经功能缺损无明显差异（P〉0.05）;2）免疫组化法检测及Western blot法检测脑缺血边缘区VEGF的表达均提示：第3、7、14天干细胞组、联合组VEGF阳性表达均高于模型组（P〈0.01）,联合组在第7、14天VEGF阳性表达高于干细胞组（P〈0.05）,联合组和干细胞组在第3天VEGF的表达无差异（P〉0.05）。结论肾脑复元汤联合人脐带间充质干细胞移植能显著改善MCAO大鼠神经功能损伤,促进脑功能的恢复。其发挥对脑的保护作用的机制可能是通过提高内源性VEGF的表达。

英文摘要：

Objective： To study the nerve protecting function of Shennao Fuyuan Tang combined with transplantation of human umbilical cord mesenchymal stem cells（HUC-MSCs） on the cerebral ischemic rats. Methods：MCAO models of rats were made and randomly divided into the model group（n = 36）,HUC-MSCs transplantation group（HUC-MSCs group）（n = 36） and Shennao Fuyuan Tang combined with HUC-MSCs transplantation group（the combined group）（n = 36）,and these groups were separately divided in to another 3 groups by execution time（3^rd,7^th,14^thday）. The Neurological Severity Scores（NSS） were observed,and the expression of VEGF was detected with immunohistochemical staining and Western blot methods. Results： 1）NSS on 3^rd,7^thand 14 thday of both HUC- MSCs group and the combined group were lower than those of the model group at the same time section（P〈0.01）. NSS of the combined group on 7^thand 14 thday were lower than those of the HUC-MSCs group at the same time section（P〈0.05 or P〈0.01）. There was no significant difference in NSS between the combined group and the HUC-MSCs group in 3^rd day（P〉0.05）. 2）The expression of VEGF in edge area of cerebral ischemia with immunohistochemical staining and Western blot methods to detect showed that the expression of VEGF of both the HUC-MSCs group and the combined group on on 3^rd,7^thand 14 thday was higher than that of the model group（P〈0.01）. The expression of VEGF of the combined group on 7^thand 14 thday was higher than that of the HUC-MSCs group at the same time section（P〈0.05）. There was no significant difference in the expression of VEGF between the combined group and the HUC-MSCs group in 3^rd day（P〉0.05）. Conclusion： Shennao Fuyuan Tang combined HUC MSCs transplantation can significantly improve the nerve function damage of MCAO rats,and promote the recovery of brain function. It plays the role of the protection of brain mechanism probably by enhancing endogenous VEGF expression.