中文摘要：

根据陆地棉GhTCP15基因序列,设计1对引物,通过PCR技术从海岛棉品种‘新海21号’中克隆一个同源基因,命名为GbTCP15。海岛棉GbTCP15基因具有一个1 056bp开放阅读框,编码351个氨基酸,预测分子量约为38 057.4kD,等电点为9.01,序列中含有一个高度保守的TCP结构域。氨基酸序列对比表明,海岛棉GbTCP15蛋白与其他植物中的TCP蛋白有较高的一致性,说明该基因在进化过程中是相当保守的。亚细胞定位结果显示,GbTCP15主要分布在细胞核上,推测GbTCP15在复制和转录的过程中发挥着信号转导以及转录调控等作用。进化树分析表明,海岛棉GbTCP15基因与雷蒙德氏棉GrTCP15基因分布在同一分支上。实时荧光定量PCR表明,海岛棉GbTCP15基因在茎部和15d纤维中表达量较高。研究表明,GbTCP15转录因子可能参与棉花纤维以及表皮毛的发育。

英文摘要：

According to the coding sequence of GhTCP15 gene,a pair of primers were designed. The homol- ogous sequence of GbTCP15 gene, was cloned from cotton（Gossypium barbadense L. ）cultivar Xinhai 21 using reverse transcription PCR methods in this study. GbTCP15 gene contained open reading frame （ORF） of 1 056 bp in length, 351 amino acids residues with a predicted molecular mass of about 38 057.4 kD and a basic isoelectric point of 9.01, with a highly conservative TCP domain in the encoded putative protein. Amino acid sequence alignment revealed of GbTCP15 protein shared high degree of identity with other higher plant TCP proteins and the gene in evolution has proved to be conservative. Subcellular locali- zation showed that GbTCP15 expressed possibly in cell nucleus. Thus, we speculated that GbTCP15 play a role on signal transduction and transcriptional regulatory during replication and transcription. The phylo- genetic tree showed that GbTCP15 was located at the same distribute with Gossypium raimondii GrTCP15. The experment results of real time PCR exhibited that GbTCP15 had a higher expression level in stems and fibers （15 d）. Above results suggested that GbTCP15 transcription factor may be involved in the cotton fiber and table fur development.