中文摘要：

通过设置不同浓度的葡萄糖溶液和维生素C溶液分别添加到普安银鲫的孵化水体中,直至卵黄囊消失完全,探究葡萄糖和维生素C溶液分别浸泡对普安银鲫(Carassius auratus gibelio)卵黄囊仔鱼发育中脂蛋白脂酶(LPL)和肝脂酶(HL)活性的影响。葡萄糖浓度为0、5、10、15和20 g/L;维生素C浓度为0、20、25、30和35 mg/L,记录孵化时间、孵化率及仔鱼成活率,并测定了最适葡萄糖浓度组、维生素C浓度组与对照组中普安银鲫卵黄囊仔鱼发育中LPL和HL活性。结果显示:普安银鲫卵黄囊仔鱼发育中对照组与维生素C组LPL比活力与全活力呈"下降-上升"的变化趋势,HL比活力与全活力均呈上升趋势。葡萄糖组LPL和HL比活力与全活力呈上升趋势,在混合营养期与外源营养期,LPL和HL比活力与全活力显著高于对照组(P<0.05),而维生素C组LPL和HL比活力与全活力仅稍高于对照组,但HL全活力在内源营养期显著高于对照组(P<0.05)。研究表明:适宜水平的葡萄糖溶液可通过调节脂类代谢酶的活性来维持机体内脂质代谢的动态平衡,同时适宜水平的维生素C溶液能促进脂质代谢。

英文摘要：

In this study, we investigated how glucose and vitamin C regulated the activities of lipoprotein lipase and hepatic lipase during yolk-sac larva development of Carassius auratus gibelio (C. auratus gibelio). Glucose solutions and vitamin C solutions at different concentrations were added into the hatching water, and the treatments lasted until the yolk sac disappeared completely. The concentrations of glucose were 0, 5, 10, 15 and 20 g/L, and the concentrations of vitamin C were 0, 20, 25, 30 and 35 mg/L. We recorded the incubation time, the hatching rate and survival rate of larvae, and tested the activities of lipoprotein lipase and hepatic lipase after the treatments with the optimal glucose level, the optimal vitamin C level, and the control. The results showed that the specific activity and total activity of lipoprotein lipase displayed a “down-up” trend in the control group and the vitamin C group, while those of hepatic lipase maintained a pattern of increase during yolk-sac larva development of C. auratus gibelio. In the glucose group, the specific activity and total activity of lipoprotein lipase and hepatic lipase were increased and significantly higher than those in the control group during the mixed nutrient stage and exogenous nutrient stage (P<0.05). In the vitamin C group, the specific activity and total activity of lipoprotein lipase and hepatic lipase were only slightly higher than those in the control group, but the total activity of hepatic lipase was significantly higher than that in the control group during the endogenous nutrient stage (P<0.05). Our studies showed that glucose solution (15 g/L) could help regulate lipid metabolic enzyme activity thus maintain the dynamic balance of lipid metabolism, and vitamin C solution (30 mg/L) could improve lipid metabolism.