中文摘要：

目的研究糙海参（Holothuria scabraJaeger）具有生物活性的三萜皂苷类成分。方法应用大孔树脂柱色谱、正相硅胶柱色谱、反相硅胶柱色谱和HPLC对糙海参体内三萜皂苷活性成分进行分离纯化,根据化合物的理化性质和波谱数据鉴定其结构。结果分离并鉴定了5个三萜皂苷类化合物,分别为：scabraside C（1）、holothurin A1（2）、echinoside A（3）、24-dehydroechioside A（4）和holothurin A（5）,化合物1～5对人白血病细胞HL-60、MOLT-4和人肺癌细胞A-549的抑制率较强。结论化合物1～5为首次从糙海参中分离得到,其中化合物1为新化合物。5个化合物均显示显著的体外细胞毒活性。

英文摘要：

Aim To study the bioactive triterpene glycosides of sea cucumber Holothuria scabra Jaeger.Methods Triterpene glycosides in the sea cucumber were isolated and purified by column chromatography on DA-101,silica gel,reversed-phase silica and reversed HPLC.Their chemical structure were identified on the basis of chemical evidence and spectral data.The cytotoxicities of the compounds obtained were tested with HL-60 cells and MOLT-4 cancer cells.Results Five triterpene glycosides were obtained from the sea cucumber studied and their structures were identified as scabraside C（1）,holothurin A1（2）,echinoside A（3）,24-dehydroechinoside A（4） and holothurin A（5）.Their inhibition activities on HL-60,MOLT-4 and A-549 cells were significant.Conclusion Compounds 15 are obstained from Holothuria scabra Jaeger for the first time.All glycosides good show antitumor activity in vitro.