中文摘要：

冷盐溶液的 intravascular 注入有效地导致的背景本地降低体温在中风减少大脑损坏。我们进一步在我们的学习决定了本地降低体温的最佳的温度。78 只成年男 Sprague Dawley 老鼠(260-300 g ) 随机被划分成 3 个组的方法：组 A，没有冷盐的注入(n=-26 )( 控制组) 的 ischemia/reperfusion；组 B，注入与 20 在灌注(n=26 ) 前盐；组织 C：有在灌注(n=26 ) 前盐的 10 ~ C 的注入。在每个组，我们选择了 15 只老鼠因为监视物理索引和大脑(外皮和 striatum ) 和身体(肛门) 的温度，大脑梗塞体积的测量，对神经病学的赤字的评价和幸存在 48 个小时灌注评价。从各个的 8 只老鼠组织的另外一个为在灌注以后在 48 个小时检验大脑浮肿， 3 由电子显微镜学(他们) 为组织学的观察从各个组织的另外一个和轻显微镜学(LM ) 被选择。在那里的结果不在考试期间是在为物理索引的 3 个组之中的重要差别(F (2,45 )=0.577， P=0.568；F (2， 45 )= 0.42，为血压的 P=0.78 和血气体分析，分别地) 。大脑温度显著地与另外的组相比在组 C 被减少(F (2， 45 )=37.074， P=0.000；F (2， 45 )=32.983， P=0.000 外皮和 striatum 温度分别地) 当在在在灌注以后的组 A 和 B 或 C 之间的直肠的温度的差别不是重要的时(F (2， 45 )= 0.17115， P=0.637 ) 。并且大脑梗塞体积显著地在组 C 被减少(从 40%?? 獲有 ?? ″ ?栠灡潬祴数 4

英文摘要：

Background Local hypothermia induced by intravascular infusion of cold saline solution effectively reduces brain damage in stroke. We further determined the optimal temperature of local hypothermia in our study. Methods Seventy-eight adult male Sprague Dawley rats （260-300 g） were randomly divided into 3 groups： group A, ischemia/reperfusion without cold saline infusion （n=26） （control group）; group B, infusion with 20℃ saline before reperfusion （n=26）; group C： infusion with 10℃ saline before reperfusion （n=26）. In each group, we chose 15 rats for monitoring physical indexes and the temperature of the brain （cortex and striatum） and body （anus）, measurement of brain infarction volume, assessment of neurological deficits and the survival rate of reperfusion at 48 hours. Another 8 rats from each group was chosen for examining brain edema, another 3 from each group for histological observation by electron microscopy （EM） and light microscopy （LM） at 48 hours after reperfusion. Results There was no significant difference among the 3 groups for physical indexes during the examination （F（2. 45）= 0.577, P=0.568; F（2.45）= 0.42, P=0.78 for blood pressure and blood gas analysis, respectively）. The brain temperature was significantly reduced in the group C compared to the other groups （F（2.45）=37.074, P=0.000; F（2.45）=32.983, P=0.000, for cortex and striatum temperature respectively）, while the difference in rectal temperature between group A and B or C after reperfusion was not significant （F（2.45）= 0.17115,P=0.637）. And the brain infarct volume was significantly reduced in group C （from 40%±10% in group A, 26%±8% in group B, to 12%±6% in group C, F（2.45）=43.465, P=0.000） with the neurological deficits improving in group C （Х^2=27.626, P=0.000）. The survival rate at 48 hours after 10℃ and 20℃ saline reperfusion was increased by 132.5% and 150%, respectively, as compared to the control group （Х^2=10.489, P=0.005）. The exten