中文摘要：

背景：前期实验发现枸杞叶主要成分枸杞多糖对成年去势雌性大鼠骨质疏松有明显的骨质改善作用。目的：观察宁夏枸杞叶对去势大鼠血清雌激素和骨组织雌激素受体表达的影响。方法：SD雌性未孕6月龄大鼠32只,分为3组。模型组和治疗组切除大鼠卵巢建立绝经后骨质疏松症动物模型。假手术组仅切除卵巢周围少许脂肪组织立即关闭切口。造模后8周后治疗组大鼠随机分为2组,分别饮水服枸杞叶500 mg/kg（治疗低剂量组）和枸杞叶1 000 mg/kg（治疗高剂量组）,每天1次,连续12周;模型组和假手术组给予等量的蒸馏水。用放射免疫法（RIA法）检测大鼠血清中的雌二醇的水平变化,运用免疫组织化学方法检测雌性大鼠各组骨组织中雌激素受体的变化。结果与结论：与假手术组比较,模型组大鼠血清雌二醇明显降低（P〈0.01）;与模型组比较,药物治疗组可以明显提高血清雌二醇浓度（P〈0.01）,高剂量组明显。与假手术组比较,模型组大鼠血清雌激素受体α、雌激素受体β明显降低（P〈0.01）,模型组比较,治疗组雌激素受体α、雌激素受体β表达量增加,差异有显著性意义（P〈0.01）。结果说明,宁夏枸杞叶能提高去卵巢骨质疏松模型大鼠血清雌二醇水平和骨细胞雌激素受体α、雌激素受体β的表达,可能是治疗骨质疏松症的重要机制。

英文摘要：

BACKGROUND： Lycium barbarum polysaccharide, the main active component of leaves of lycium barbarum, has a remarkable therapeutic effect on osteoporosis in adult ovariectomized female rats. OBJECTIVE： To explore effects of leaves of Lycium barbarum on serum estradiol and bone estrogen receptor expressions in adult oxariectomized female rats.METHODS： Thirty-two 6-month-od female rats were randomized into sham-surgery, model, and treatment groups, respectively. Rat models of postmenopausal osteoporosis were induced by ovariotomy followed by orally administration of water solution of leaves of Lycium barbarum（500 or 1 000 mg/kg） or distilled water once daily for consecutive 12 weeks in treatment and model groups, respectively. Serum estradiol level was detected by radiation immunoassay method. Estrogen receptor α, β immunoreactivities in bone tissue were determined by immunohistochemical staining.RESULTS AND CONCLUSION： Serum estradiol level in the model group was significantly decreased compared with the sham-surgery group（P〈0.01）, but that was significantly increased in the treatment groups, particularly at high dose, compared with the model group（P〈0.01）. Estrogen receptor α, β immunoreactivities were weaker in the model group than the sham-surgery group（P〈0.01）, and those were stronger in the treatment groups than the model group（P〈0.01）. Our results suggest that leaves of Lycium barbarum treat osteoporosis through enhancing serum estradiol and estrogen receptor expressions in adult ovariectomized rats.