中文摘要：

为研究海洋微生物间降解高分子量多环芳烃（High molecular weight - polycyclic aromatic hydrocarbons，简称HMW．PAHs）过程中的相互作用，筛选具有协同效应的菌群，以1株可降解HMW-PAHs的解环菌属细菌Cycloclasticussp．PY97M和4株其他属细菌分别构建二元菌群降解芘和荧蒽，通过GC-MS测定HMW-PAHs降解率，并采用发光细菌法测定其降解前后的生物毒性．结果表明，由PY97M和Marinobacter nanhaiticus D15-8W^T组成的菌群（PY97M＋D15-8W）对初始质量浓度均为0．1g／L的单一碳源芘、荧蒽21d后的降解率分别为67．40％和62．79％，相对于纯培养PY97M分别提高了20．30％和20.29％．另外，该菌群对初始质量浓度均为0．1g／L的芘和荧蒽组成的混合碳源14d后的降解率分别为71．05％和67．36％；生物毒性检测结果显示，HMW-PAHs经该菌群降解后其急性毒性和遗传毒性相对于母体都有显著降低．解环菌与海杆菌在芘和荧蒽的降解过程中表现出明显的协同效应，表明该菌群具有应用到HMW-PAHs污染海洋环境生物修复的潜力．图5表1参24

英文摘要：

In order to investigate the interaction between marine microorganisms and screen the synergic consortium in the biodegradation of high molecular weight polycyclic aromatic hydrocarbons （HMW-PAHs）, we co-cultured HMW- PAHs degrader Cycloclasticus sp. PY97M with the other four bacterial species to construct defined consortia for pyrene and fluoranthene biodegradation. The degradation rates were measured by gas chromatography-mass spectrometry （GC- MS） and the end-product＇s biotoxicity of PAHs measured by photobacterium test. Pyrene and ftuoranthene, each at an initial concentration of 0.1 g/L, was supplemented as sole source of carbon and energy for the growth of the defined consortia throughout a 21-day incubation. The degradation rates by the consortium composed of Cycloclasticus sp. PY97M and Marinobacter nanhaiticus D15-8Wr could reach 67.40% and 62179% for pyrene and fluoranthene respectively, which was 20.30% and 20.29% higher than treatment of the PY97M pure culture. The consortium could utilize 71.05% of pyrene and 67.36% of fluoranthene respectively throughout a 14-day incubation, in which the mixed carbon source composed of pyrene and fluoranthene was each at an initial concentration of 0.1 g/L. The results of the biotoxicity test also indicated a significant reduction of the acute toxicity and geno-toxicity after HMW-PAHs biodegradation compared with the parent chemicals. The prominent synergic effect of Cycloclasticus strains and M. nanhaiticus strains suggested a great application potential in marine HMW-PAHs bioremediation. Fig 5, Tab 1, Ref24