中文摘要：

脂质体作为一种药物基因载体已得到广泛应用，然而其仍然具有物理化学稳定性差、易发生团聚、难以多功能化等缺点．通过使用合成的双亲性高分子共轭亚油酸修饰聚赖氨酸（PC）代替小分子磷脂制备的高分子脂质体（PLs），不仅保留了脂质体的优势，并且克服了上述缺点；通过对高分子进行聚乙二醇（PEG）修饰，可使制备的高分子脂质体具有长循环性．结果表明，高分子脂质体粒径为纳米级，具有药物缓释性能、较低的细胞毒性及较高的细胞内吞效率．

英文摘要：

Recently, liposomes have gained attention as a promising tool for drug and gene delivery. However, their applications have been constrained by their poor stability, aggregation and difficult to functional. Using amphiphilc conjugated linoleic acid modified polylysine （PC） and cholesterol, we developed a novel polymeric liposomes （PLs）. These PLs retained the advantages of conventional liposomes （CLs）, and overcome the above disadvantages of CLs. In addition, PEG chains coated on the PLs surface can prolong their circulation time in the blood. These results suggest that the PLs were nano-sized, achieved a sustained release of drugs, showed limited cytotoxicity and increased uptake in LN229 glioblastoma cells.