中文摘要：

利用高效毛细管区带电泳,建立一种同时分离测定茶叶中儿茶素、表儿茶素、槲皮素、山奈酚和杨梅素的方法.在含有10 mmol/L Na2B4O7、5 mmol/Lβ-环糊精及8％（V/V）乙腈的运行缓冲溶液（pH 9.11）中,采用20 kV的分离电压、25℃的毛细管柱温和200 nm的检测波长,儿茶素、表儿茶素、槲皮素、山奈酚和杨梅素可以在14 min实现有效分离与检测,将方法用于不同茶叶样品中这5个组分的测定,相对标准偏差在4.0％以内,回收率为95.4％～104.6％.本方法简单、快速、线性范围较宽、重复性好,可用于茶叶中这5个组分的测定.

英文摘要：

Objective：To develop a method for simultaneous separation and determination of catechin,epicatechin,quercitin,kaempferol and myricetin in tea.Methods：High performance capillary zone electrophoresis was used.Results：Catechin,epicatechin,quercitin,kaempferol and myricetin could be effectively separated and determined within 14 min in a running buffer solution （pH 9.11） containing 10 mmol/L Na2B4O7,5 mmol/L β-CD and 8％ （V/V） acetonitrile with the applied voltage of 20 kV,capillary temperature of 25 ℃ and detector wavelength of 200 nm.The relative standard deviation and recovery of the proposed method for these five compounds in tea were below 4.0％ and in the range of 95％-105.6％,respectively.Conclusion：This method possesses the advantages of simple operation,rapid determination,high reproducibility and wide detection range.It can be used for the determination of catechin,epicatechin,quercitin,kaempferol and myricetin in tea.