中文摘要：

目的：优化潞党参核苷类成分的提取工艺。方法：采用超声提取法提取潞党参中核苷类成分,以胞苷、尿苷、鸟苷和腺苷总量为评价指标,以乙醇体积分数（包括水）、提取时间、料液比、提取次数为考察因素,结合单因素和正交试验,对提取工艺条件进行筛选和优化,并进行验证试验。结果：最优提取工艺为以水为提取溶剂,提取时间45 min,料液比1∶20（g/ml）,提取2次;验证试验中潞党参100 g中4种核苷类成分总量为（17.01±0.005）mg（RSD=0.68%,n=3）。结论：优化的潞党参总核苷提取工艺稳定可行,且操作快速、节约成本。

英文摘要：

OBJECTIVE：To optimize the extraction technology of nucleoside from Codonopsis pilosula.METHODS：The ultrasonic extraction was used to extract nucleoside from C.pilosula.The extraction technology was screened and optimized by single factor and orthogonal test with ethanol concentration（including water）,extraction time,solid-liquid ratio and extraction times as factors using the total contents of cytidine,uridine,guanosine and adenosine as index.Validation test was conducted.RESULTS：The optimal extraction technology was that pure water was extraction solvent,extracting for 45 min,with material-liquid ratio of 1 ∶20（g/ml）,extracting for 2 times.Total amount of 4 kinds of nucleosides from in 100 g C.pilosula was（17.01±0.005）mg（RSD=0.68%,n=3）.CONCLUSIONS：Optimized extraction technology of nucleoside from C.pilosula is stable,feasible,rapid in operation and cost-saving.