中文摘要：

从一个专业化 cDNA 图书馆，四新奇盒子 H/ACA snoRNAs，命名 GLsR22， GLsR23， GLsR24 和 GLsR25，从原始优核质被识别， Giardia lamblia.Bioinformatics 分析显示所有能潜在地他们被合拢进两倍发卡，盒子 H/ACA snoRNAs 的典型第二等的结构。当时， GLsR24 和 GLsR25 被预言分别地，在 23S rRNA 上在 U1753 和 U2396 指导 thesite 特定的 pseudouridylation GLsR22 andGLsR23 属于家庭使 snoRNAs “变成孤儿”。所有被单个拷贝基因四新奇 snoRNAs 编码并且定位了在小内部遗传因子的区域。与以前在 Archaea 和另外的单细胞的原生动物门报导的对应物相比，有趣地，盒子 H/ACA snoRNAs 从 G 识别了。lamblia 有唯一的结构的特征，暗示那 snoRNAs 以不同方法从初核质演变到优核质。

英文摘要：

From a specialized cDNA library, four novel box H/ACA snoRNAs, named GLsR22, GLsR23, GLsR24 and GLsR25, were identified from the primitive eukaryote, Giardia lamblia. Bioinformatics analyses indicated that all of them can be potentially folded into double hairpins, the typical secondary structures of box H/ACA snoRNAs. GLsR24 and GLsR25 are predicted to guide the site-specific pseudouridylation at U1753 and U2396 on 23S rRNA respectively, while GLsR22 and GLsR23 belong to the family of ＂orphan＂ snoRNAs. All of the four novel snoRNAs are encoded by single copy genes and located in small intergenic regions. Interestingly, compared with the counterparts previously reported in Archaea and other unicellular protozoan, the box H/ACA snoRNAs identified from G. lamblia have unique structural features, implying that snoRNAs evolved from prokaryotes to eukaryotes in different ways.