中文摘要：

目的：运用MADB-106大鼠乳腺癌细胞系制备胫骨癌痛模型。 方法：实验于2005-03／12在华中科技大学同济医学院附属同济医院麻醉学实验室完成。选择雌性SD大鼠20只，随机分为对照组和模型组，各10只。按照Medhurst等报道的方法制作胫骨癌痛模型。大鼠麻醉后仰卧位捆绑，从左侧胫骨上部切开皮肤，分离肌肉，暴露胫骨，使用20mL注射器针头穿刺打孔，之后换上10μL注射器进入骨髓腔，缓慢注入3μL MADB-106大鼠乳腺癌细胞（4．8×10^9L^-1），注射完毕后用骨蜡封住针孔，皮肤缝合。对照组动物左侧胫骨上段骨髓腔注入等体积Hank液，其余操作同模型组。①机械性痛觉超敏：使用37400-002型接触刺激仪测定大鼠足底缩爪阈值。造模前测定足底基础痛阈值，从次日开始连续测痛，将刺激仪细纤维接触大鼠左侧足底中部，在5s内最大可追加至50g力量，观察大鼠缩爪反应并记录缩爪阈值。每只动物测试5次，两次测试至少间隔5min。②辐射热痛：使用BME-410A型辐射热测痛仪评价大鼠对热刺激的反应，将大鼠置于玻璃板上，使用热辐射仪照射其后趾相应的部位，记录从照射到缩爪反应的时间，即缩爪潜伏期。在实验前均先测定足底基础痛阈值，测量同一部位时，每次间隔5min，以使其痛觉恢复正常，每只大鼠左右足底各测试3次，缩爪时间即为其痛阈值。两组于术后8，14，22d，将大鼠麻醉后术侧后肢进行X射线摄片和苏木精-伊红染色观察两组术后骨质破坏情况。 结果：纳入动物20只，均进入结果分析。①疼痛行为学观察：对照组大鼠对机械痛和辐射热痛刺激的缩爪阈值在术后各时间点组内相比差异无显著性，而模型组大鼠机械痛刺激与对照组大鼠在术后的前12d缩爪阈值相比差异无显著性，术后14～22d，两组缩爪阈值相比差异显著（P〈0．05）；模型组大鼠辐?

英文摘要：

AIM： To establish models of tibial cancer pain with MADB-106 mammary gland carcinoma cell line （CCL） of rats. METHODS： The experiment was conducted in the Department of Anesthesiology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology from March to December 2005. Twenty female SD rats were randomly divided into control group and model group with 10 rats in each group. Models of tibial bone cancer pain were made according to the method described by Medhurst et al. Following anesthesia, rats were placed with abdominal sides up. An incision was made in skin over top half of left tibia, and muscles were separated to expose the tibia. A 20 mL syringe needle was used to perforate the tibial plateau, and then 3 μL MADB-106 mammary gland carcinoma cells of rats （4.8 ×10^9 cells/μL） was injected slowly into cavitas medullaris with a 10 μL syringe. Bone wax was used to seal the pinhole as soon as injection, and wound was sutured immediately. Rats in the control group were injected with Hank balanced salt solution into the top segment of cavitas medullaris in left tibia at the same volume, rest procedures were the same as those in the model group.①Mechanical allodynia： Mechanical withdrawal thresholds of rats＇ hind paws were measured by using 37400-002 Dynamic Plantar Aesthesiometer. Withdrawal thresholds were measured before modeling, and measurements were conducted successively from the next day. Small fibers of aesthesiometer were contacted with middle part of left paws with the maximum power of 50 g within 5 seconds; Withdrawal of rats were observed and the thresholds were recorded. Each rat was measured 5 times and there were at least 5 minutes in each interval. ②Radiant heat threshold： Rat＇s reaction to thermal stimulation was nleasured and evaluated by using BME-410A thermal dolorimeter, rats were placed on the glass plates with their hind toes exposed to thermal radiometers; Time from radiation to withdrawal i.e. latencies were recorded.