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中文摘要：
为研究红景天甙（salidroside）对β淀粉样肽25-35（β amyloid peptide25-35，Aβ25-35）诱导PC12细胞凋亡的抑制作用，采用Cell Counting Kit-8（CCK-8）分析细胞的存活率，通过光镜检测细胞形态并配以Hoechst染色检测细胞核固缩，利用荧光共振能量转移（fluorescence resonance energy transfer,FRET）技术在单个活细胞中检测caspase-3和caspase-8活性的动态变化。结果表明，红景天甙可剂量依赖性抑制Aβ25-35引起的细胞凋亡，提高细胞的存活率；红景天甙对caspase-3的活性有明显的抑制作用，而且Aβ25-35诱导细胞凋亡不依赖于caspase-8的激活。这些结果提示抑制caspase-3的活性是红景天甙抑制Aβ25-35诱导PC12细胞凋亡的机制之一。
英文摘要：
To investigate the inhibitions of salidroside on the apoptosis induced by Aβ25-35 in PC12 cells, cell survival was analyzed with cell counting Kit-8 （CCK-8）, cell morphology was observed by optical microscope, nuclear condensation was shown by Hoechst staining, the dynamics of caspase-3 and caspase-8 activation in single living cell were monitored by fluorescence resonance energy transfer （FRET） respectively. The results showed that salidroside elevated the cell survival by inhibiting the Aβ25-35-induced apoptosis in a dose-dependent manner, and that salidroside greatly inhibited the caspase-3 activation. Furthermore, the apoptosis induced by Aβ25-35 was independent of the caspase-8 activation. In conclusion, these data indicate that salidroside can protect the PC12 cells from Aβ25-35-induced apoptosis by inhibiting the activation of caspase-3.
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