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锌影响辣椒素致痛模型小鼠脊神经节P物质表达和热痛阈的实验研究

ISSN号：1006-2947
期刊名称：《解剖科学进展》
时间：0
分类：R337.5[医药卫生—人体生理学;医药卫生—基础医学]
作者机构：[1]辽宁省锦州市中医医院普外科, [2]辽宁医学院组织胚胎学教研室,辽宁锦州121000
相关基金：辽宁省科技计划项目（No.2013225305）; 辽宁省大学生创新训练计划项目（201210160007）; 辽宁医学院横向基金（LYHX2013025,2013024）; 奥鸿博泽研究生科研创新基金（2012009）

作者：张晓杰, 刘晓露[2], 李富兴[2], 韩言秀, 王月静[2], 张莉[2]

关键词：锌, 神经病理性疼痛, 脊神经节, P物质, 热痛阈, 小鼠, zinc, neuropathic pain, dorsal root ganglian, substance P, thermal pain threshold, mouse

中文摘要：

目的研究锌对辣椒素致痛模型小鼠脊神经节P物质（substance P,SP）表达和热痛國的影响。方法 48只C57/BL6小鼠随机分4组,采用右后足底注射辣椒素（0.5%,5μ1）制备神经病理性疼痛（neuropathic pain,NPP）模型：对照组和适锌喂养组给予适锌饲料（锌30mg/kg/d）;低锌喂养组给予低锌饲料（锌0.85mg/kg/d）;高锌喂养组（H-NPP）给予硫酸锌水溶液（227mg/L/d）。2周后,对照组注射溶剂（吐温80、酒精和生理盐水混合液）,其它三组注射辣椒素。应用金属自显影技术、热痛國检测、免疫组织化学和图像分析技术检测注射后7d锌对脊神经节SP表达和热痛國的影响。结果高锌喂养显著提高了脊神经节中锌的含量,降低SP的表达,使热痛國增高,痛敏减弱（P〈0.01）;而低锌喂养能使脊神经节的锌更少,SP的表达更高,热痛國更低,痛敏更强（P〈0.01）。结论锌能抑制辣椒素致痛模型小鼠脊神经节SP的表达,使热痛國升高,痛敏降低。

英文摘要：

Objective To study the effect of zinc on the expression of substance P （SP） in dorsal root ganglian and the thermal pain threshold of capsaicin-induced neuropathic pain（NPP） in mice. Methods 48 C57/BL6 mice were divided into 4 groups randomly, then capsaicin was injected in the right hind foot of mice to build the NPP mice model. Mice in control group and N-NPP group were fed by normal food, in L-NPP group fed by low-zinc food, in H-NPP group fed by high-zinc food. Then the control group were injected with solvent （Tween 80, alcohol and saline）, while the other group were injected with capsaicin 2 weeks later. Autometallography, thermal pain threshold observation, immunohistochemistry and image analysis were used to examine the effect of zinc on the expression of SP in dorsal root ganglian and the thermal pain threshold at the 7th day after injection. Results High-zinc feed can increase zinc level in dorsal root ganglian and the thermal pain threshold, downregulate the SP expression level（P〈 0.01）. Low-zinc feed can reduce the zinc level in dorsal root ganglian and the thermal pain threshold, upregulate the SP expression level（P〈 0.01）. Conclusion Zinc can inhibit the SP expression in dorsal root ganglion and improve the thermal pain threshold in capsaicin-induced NPP model mice.

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